LOCUS       sequence-136288-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a guide RNA (gRNA) to target human IRF-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Fujita T, Fujii H
  TITLE     Efficient isolation of specific genomic regions and identification 
            of associated proteins by engineered DNA-binding molecule-mediated 
            chromatin immunoprecipitation (enChIP) using CRISPR.
  JOURNAL   Biochem Biophys Res Commun. 2013 Sep 13;439(1):132-6. doi: 
            10.1016/j.bbrc.2013.08.013. Epub 2013 Aug 11.
  PUBMED    23942116
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2013.08.013"; journalName: "Biochem Biophys Res 
            Commun"; date: "2013-09-13- 13"; volume: "439"; issue: "1"; pages: 
            "132-6"
COMMENT     Sequence Label: gRNA-hIRF-1 #12
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(131..136,137..154,155..161)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    169..185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     174..196
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     193..209
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     193..209
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        227..245
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     227..244
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    330..354
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(366..441)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(462..710)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(520..539)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(690..710)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(849..868)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(850..868)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(875..892)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(875..891)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(884..906)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      181 aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt aggtgacact
      241 atagaatact caagctatgc atcaagcttg gtaccgagct cggatccact agtaacggcc
      301 gccagtgtgc tggaattcgc cctttaatgc caactttgta caagaaagct gggtctagaa
      361 aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattttaact
      421 tgctatttct agctctaaaa cggacagccc agcgcccccg cggtgtttcg tcctttccac
      481 aagatatata aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc
      541 attttaaaac ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac
      601 gtattttgta ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca
      661 gccttgtatc gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg
      721 accttggtac cggatccagt cgactgaatt ggttccttta aagcctgctt ttttgtacaa
      781 agggcgaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca tctagagggc
      841 ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
      901 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      961 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat
     1021 acgtacggca gtttaaggtt tacacctata aaa
//
