LOCUS       sequence-136315-         512 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gi-coupled hM4D DREADD fused with mCherry under the 
            control of human synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, 
            Maratos-Flier E, Roth BL, Lowell BB
  TITLE     Rapid, reversible activation of AgRP neurons drives feeding behavior
            in mice.
  JOURNAL   J Clin Invest. 2011 Apr;121(4):1424-8. doi: 10.1172/JCI46229.
  PUBMED    21364278
REFERENCE   2  (bases 1 to 512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Clin Invest"; date: "2011-04"; volume: "121"; issue: "4"; pages: 
            "1424-8"
COMMENT     Sequence Label: pAAV-hSyn-DIO-hM4D(Gi)-mCherry
FEATURES             Location/Qualifiers
     source          1..512
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
       61 aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct caccatggtg
      121 gcgaccggtg gatcccgcct ggcagtgccg atgttccgat actggcacag cagcaggtgc
      181 cggaaggtct ttttaaaggt ggcgttgcac agagcatagc aggcagggtt gatggtgctg
      241 ttgacgtagc agagccagta gccaatggac cacaccgtgt cagggatgca gctctggcag
      301 aaggtgttca ccaggaccat gacgttgtag ggcgtccagg tgaggatgaa ggccagcaga
      361 atggcaaaga tcgttcgtgt cactttgcgc tcccgggccg ccatctgccg cttcttgcgc
      421 acctggttgc gagcgatgct ggcgaacttg cgggccacgt tggccgcagg gcgcatgcca
      481 gccggcgtgg caggcacaat ctcaatggct gt
//
