LOCUS       sequence-136421-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
  TITLE     Engineering a Circular Riboregulator in Escherichia coli.
  JOURNAL   Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. 
            eCollection 2020.
  PUBMED    37849901
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biodes 
            Res."; date: "2020-09-12"; volume: "2020:1916789. doi"; pages: " 
            10.34133/2020/1916789. eCollection 2020"
COMMENT     Sequence Label: cisRAJ31-cat
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    55..74
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     protein_bind    108..124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             171..182
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(255..274)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtgagccag tgagttgatt gctacgtaat tagttagtta gcccttagtg actcgaattc
       61 gcggccgctt ctaggatgca tataaatgtg agcggataac attgacattg tgagcggata
      121 acaagatact gagcacagtg gatatgcttt acctcttggt gagacgacat aaagaggaga
      181 aacataatat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      241 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
      301 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
      361 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga
      421 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
      481 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
      541 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
      601 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
      661 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
      721 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtt
//
