LOCUS       sequence-136434-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for Citrine purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pET21a(+)-Histag-Citrine (525)
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(77..793)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(800..817)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttgtcgacg gagctctcat ttgtacaatt catccatacc atgggtaata ccagcagcag
      121 taacaaattc taacaagacc atgtgatctc tcttttcgtt tggatctttg gataatctag
      181 attgatagga taagtaatgg ttgtctggta acaagactgg accatcacca attggagtat
      241 tttgttgata atggtcagct aattgaacag aaccatcttc aatgttgtgt ctaattttga
      301 agttaacttt gataccattc ttttgtttgt cagccatgat gtaaacattg tgagagttat
      361 agttgtattc caatttgtga cctaaaatgt taccatcttc tttaaaatca atacctttta
      421 attcgattct attaactaag gtatcacctt caaacttgac ttcagctctg gtcttgtagt
      481 taccgtcatc tttgaaaaaa atagttcttt cttgaacata accttctggc atggcagact
      541 tgaaaaagtc atgttgtttc atatgatctg ggtatctagc aaaacacatc aaaccataac
      601 ctaaagtagt gactaaggtt ggccatggaa ctggcaattt accagtagta caaataaatt
      661 ttaaggtcaa tttaccgtaa gtagcatcac cttcaccttc accggagaca gaaaatttgt
      721 gaccattaac atcaccatct aattcaacca aaattgggac aacaccagtg aataattctt
      781 cacctttaga catggatccg tgatggtgat ggtgatgcga tcc
//
