LOCUS       sequence-136494-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Lambda Red recombinases and a dominant negative MutL 
            allele all controlled by temperature sensitve cI857 repressor for 
            high precision and efficiency MAGE experiments. Cam resistance 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Nyerges A, Csorgo B, Nagy I, Balint B, Bihari P, Lazar V, Apjok G, 
            Umenhoffer K, Bogos B, Posfai G, Pal C
  TITLE     A highly precise and portable genome engineering method allows 
            comparison of mutational effects across bacterial species.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16. pii: 201520040.
  PUBMED    26884157
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 16. pii: 201520040."
COMMENT     Sequence Label: pORTMAGE-4
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgagcgacc tgcgtcggta gtcaaagaac tagtgaaaaa cagcctcgat gcaggtgcga
       61 cgcgtatcga tattgatatc gaacgcggtg gggcgaaact tatccgcatt cgtgataacg
      121 gctgcggtat caaaaaagat gagctggcgc tggcgctggc tcgtcatgcc accagtaaaa
      181 tcgcctctct ggacgatctc gaagccatta tcagcctggg ctttcgcggt gaggcgctgg
      241 cgagtatcag ttcggtttcc cgcctgacgc tcacttcacg caccgcagaa cagcaggaag
      301 cctggcaggc ctatgccgaa gggcgcgata tgaacgtgac ggtaaaaccg gcggcgcatc
      361 ctgtggggac gacgctggag gtgctggatc tgttctacaa caccccggcg cggcgcaaat
      421 tcctgcgcac cgagaaaacc gaatttaacc acattgatga gatcatccgc cgcattgcgc
      481 tggcgcgttt cgacgtcacg atcaacctgt cgcataacgg taaaattgtg cgtcagtacc
      541 gcgcagtgcc ggaaggcggg caaaaagaac ggcgcttagg cgcgatttgc ggcaccgctt
      601 ttcttgaaca agcgctggcg attgaatggc aacacggcga tctcacgcta cgcggctggg
      661 tggccgatcc aaatcacacc acgcccgcac tggcagaaat tcagtattgc tacgtgaacg
      721 gtcgcatgat gcgcgatcgc ctgatcaatc acgcgatccg ccaggcctgc gaagacaaac
      781 tgg
//
