LOCUS       sequence-136495-         607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Lambda Red recombinases and a dominant negative MutL 
            allele all controlled by temperature sensitve cI857 repressor for 
            high precision and efficiency MAGE experiments. Cam resistance 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Nyerges A, Csorgo B, Nagy I, Balint B, Bihari P, Lazar V, Apjok G, 
            Umenhoffer K, Bogos B, Posfai G, Pal C
  TITLE     A highly precise and portable genome engineering method allows 
            comparison of mutational effects across bacterial species.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16. pii: 201520040.
  PUBMED    26884157
REFERENCE   2  (bases 1 to 607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Feb 16. pii: 201520040."
COMMENT     Sequence Label: pORTMAGE-4
FEATURES             Location/Qualifiers
     source          1..607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             156..164
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    179..197
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg
       61 tgctcatacg ttaaatctat caccgcaagg gataaatatc taacaccgtg cgtgttgact
      121 attttacctc tggcggtgat aatggttgca tgtactaagg aggttgtatg gaacaacgag
      181 atgtgtataa gagacagctg gcctgcccct cccttttggt gtccaaccgg ctcgacgggg
      241 gcagcgcaag gcggtgcctc cggcgggcca ctcaatgctt gagtatactc actagacttt
      301 gcttcgcaaa gtcgtgaccg cctacggcgg ctgcggcgcc ctacgggctt gctctccggg
      361 cttcgccctg cgcggtcgct gcgctccctt gccagcccgt ggatatgtgg acgatggccg
      421 cgagcggcca ccggctggct cgcttcgctc ggcccgtgga caaccctgct ggacaagctg
      481 atggacaggc tgcgcctgcc cacgagcttg accacaggga ttgcccaccg gctacccagc
      541 cttcgaccac atacccaccg gctccaactg cgcggcctgc ggccttgccc catcaatttt
      601 tttaatt
//
