LOCUS       sequence-136558-        3152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap 3-site Destination vector for C-terminal GFP tagging with 
            embedded Cbr-unc-119 selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS235
FEATURES             Location/Qualifiers
     source          1..3152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(93..121)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    680..713
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2677..2688
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2837..2870
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     3060..3080
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccgcgaagag cggatccatg ggctcttcgg gcagcggtgg cagtggaggt accggcggaa
       61 gcggtagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      121 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      181 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tgggtaagtt
      241 taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca cttgtcacta
      301 ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa cggcatgact
      361 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      421 acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca tacatattta
      481 aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      541 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      601 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttgtaagtt
      661 taaacatgat tttactaaca taacttcgta taatgtatgc tatacgaagt tatttctaga
      721 cattctctaa tgaaaaaatc tttcagttga aattgaaaat gagttaaagt tggagttttt
      781 attgaaaaca gatttccgtg tgattagtgt ttttagcgag tgtgacagga cagcgaaaaa
      841 atatagaaac aaggggggaa ctgaaaagct taggaatgca ttgaacatga gaaggggaag
      901 gggaaggaac aaactagaca ggaattattg gaatttaatc acatttggag ttttttttct
      961 attcgacaga ataattatcc agaacatttt tgtattaaat atttatgcat catatgagta
     1021 gtcggctttg ttgtgcatga cgagtttgtt atcgacgaaa tagaagctgt cagaacgagt
     1081 ctcgtttgga ttgttgatca tgtcgtccac tgaaaaagag attagtcttt tgaattgtac
     1141 tttttagaat aatgactcac tgagttgttg agagagttga gggaactcat agatatgttc
     1201 acagttgttt cgtgaattcg gaatacagaa tccgaattca aagtcaaaac acttcagaag
     1261 gcgatctttg aagaagtgac gttcgatcat tcggaaatga tggattggga tgtctcctac
     1321 tttgaattcc acagttgctc cgaccgtttt gagtttcaaa aagtttggag caaatctata
     1381 acgcacgtat cttgccgact cctgtggcga ctcatcattc tcctgatcgt tctccggttt
     1441 ggcgatctcg aaaagcactt gctcagtgtc cagatcacgg atttggaact tggtgaactc
     1501 gatgttatag atgttcgcag atggggagca taagaatcct aaatttatgt tttaaactga
     1561 aatccaaagg gagcaagata ccttgagtga ttcccggaag tgctaaaacg tcgttcggag
     1621 tgatttgagc tttcttcgca agctccgatt ccgttgtgat tccttgttcg gtgcttggtg
     1681 gtggccgtgg catctggaaa tatggaaaag ttcaacaaaa agaaaagaga aaagaatgaa
     1741 atcggatatc aagagttagt tgagcggttt ctctagtttt ctgagtctca cctgcgacgg
     1801 gaaggtcgcc gagccgggtg gaatcgattg ttgttgctcg gctttcatat cggtttggtt
     1861 ggaagcggct gaaaacggaa agaagtggaa gaaggaaaag agtgtggtgt gacaggaaaa
     1921 tggtaattag agggtgccaa ataaccagct atattttgtt tttttttgaa aacattttta
     1981 aaaagaaaaa tacgataatg atatcagatg gatttccgga aaactggtat gaaaaatttc
     2041 aacctttttg agtacatgta atcaaaatac actttgtaaa ttatcatttt tattgaaact
     2101 ccaccatttt tctatttata acgctaataa tttgaaaaag aaacctgttg cgaaccgcgg
     2161 ggtgaatccc aaaaacgaat gcgttttggt ggagtgattg attcgaatcg aagaagaaaa
     2221 agaagaagac gtggaataga gagctcactc ttaaccgagc agcacacacc gacagaaaaa
     2281 aaaatgaaat gaatgagggt cttcttcttc ttcttcttcg aatgattgac agaaatggga
     2341 aaaagaggaa gattgagaag ggaaaaagga aggagaaaag aagcagaaga agacgtcaga
     2401 gaggagagga acgagcggaa aagcagcggg cgcaagtcat agaagtagca gagctgggga
     2461 gaagaagaca ctatccaaga aaggaatgac gagagagtat gcaaaggggt atagggtgca
     2521 gacagaatag gaacagaata acagatgatg agccaagaag agttgaaaag ggcgatgaat
     2581 ttgtcatgta acttaatttg ggtcaatttg agcatgatga attgaaatca tcccttgttg
     2641 ggagttaata accggtttgt tatcagaaac cctgtaatag aagggcgccc taactttgag
     2701 ccaattcatc ccggtttctg tcaaatatat caaaaagtgg tcaactgaca aattgttttt
     2761 gatattataa taaacatttt atccgttaac aattttcgaa tactttttac aaggacttgg
     2821 ataaattggc tcaaagataa cttcgtataa tgtatgctat acgaagttat taactaatct
     2881 gatttaaatt ttcagaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     2941 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     3001 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     3061 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     3121 tgacgaagag cggatccatg ggctcttcga ct
//
