LOCUS       sequence-136565-        5179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Punc-17::2xNLS-FLP-D5 Expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS260
FEATURES             Location/Qualifiers
     source          1..5179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3226..3250
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3259..3279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4648..4719
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
     protein_bind    complement(4723..4747)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tacaccaatc atttctcccc tccgaaatgt gtagtcgtca tgacaaagtg gtgacactgg
       61 attacggtag gctccgccca cttcttggaa aagtgacgtg ggctcggtga gccctaaagc
      121 ttttggtttt cacaattttc tggtttttgg gatttttgcg ggaattttga gaggagaaag
      181 ttgggagaga agatattgga atggtccccg gggagctgta catttataca tctagactga
      241 agctggggta ttgcaaaaag agaaaagcct atcactttct ttccatcctg catacttgcc
      301 aaaaagtttt ggctgagctt atttttaaaa ttggaatttt ttggaatttt cagaaaagaa
      361 aaagtttttt cttttttttt aatttaaaaa atttttgcta tttattgcat ggtactacag
      421 aaaaacttga atgaaataaa aaatttggaa aattcaagaa aacttttttt ttcatagttt
      481 atttattcga gttttaatgt ggcttttatt ttgttcggtt tttgttttaa aaagatttta
      541 tacaaaattt caagaattta caatttttcc gttgtaaaaa atccaaaaaa aaatccaaaa
      601 aaatagttca gacctttttt taaattttaa atttaaaaat tcaataccaa aaaattaaaa
      661 aaaattagtt tttccgagaa taaaggaaaa atgttgaaaa aatattatat ataaaatttc
      721 cagaaaatgt gaaaactcca aaaaactgca gacttttccc caaactagct attttttgaa
      781 ttcattacct ttaaaggtgg agtaccgaaa tttaagactt tgctttttta gacccaaaat
      841 ggtccaaaac taccaaattt cgtaatgaga cgttctgaaa atttttcaaa aaaatgttat
      901 gggagctcaa agtttttgaa aaaatggtat atttttagct aattttaacc acttcttggc
      961 gtcgcagcgg ttggaactca attttaatta aattatcatc cttcaatgtc cttcaatctt
     1021 tgcaatttca atcgaccata cgcgttcaga gcattttttt ttttcgtttt tcctcccgtc
     1081 cagcacatat ttaaatattc tctctccccc tcccctggtt cctaaaaact tgggaaaggg
     1141 gaatttcgaa ggaggtggaa gcttcttctt ctccccgagc acacacacac atcctctctt
     1201 caaaatcttt aagaaatagt gcgtgtgaat gactagacgc tgaagctaac ttggtttccg
     1261 cgaacaatcc caatcagtgc ctgtgccgag gcttcggaga agaagagatt gacgtcgtct
     1321 ttttcttttt ttgtgaaaca gaataaaaaa gaatacgaaa tggacttttt cacacatttt
     1381 cgttactttt ccagaaaaaa acgaagctct agccatggaa gtttgaattg aaaacctaaa
     1441 ccccgatgtg tattcatttg agcttaagcc taagcctaat tctacgccta agcataagcc
     1501 taagcctatg ttcaagccta agccaaattg tagaacgtta ccaaaagatc aaacctattt
     1561 ttcctacaga aaaggtctaa tgataactgt taagcgagag acaaggagag atatatatca
     1621 aaaactagcc caaactgtga ttaacatcta ttaactgacc tcaaaaaaaa aaatagtaga
     1681 gattaaatac tgctcaaaaa gtttcttgga aaagtaaatt gttaggtttc tggggcctgt
     1741 aaaactggac cgagttttga agaatttttt taacttttca aagtttggcg tcactgaaag
     1801 ctttatattg tacatgttcc accaattttc ttttgtatcc gttttttctg tctcaaaacg
     1861 gagctcgagc ccatgacgtg tggataccgt gagaatccgc ccaattcctt cacatcgatc
     1921 tcatatttct ttcccagttt atattgaatt cccgttgaat tgcggctttg caggggagaa
     1981 gatgtgcgtc tttatatttt tttttctatc cttacccgcc gtgaatccgg ctcgaattga
     2041 gccaattttt atgaatagta gagctaattg acttcgtctc cctagaattg atgaggtaaa
     2101 gctcgtgggg agatatggag atttttcgat tttttttcca gtaattttca gttcaaaatt
     2161 tgagcttttg agcaggagtt ttgagatttg tgccaaaatt tcctattatt ttttcaaata
     2221 tttttgcttt tgtaaaaaag gtgtagtaga agttttgtag tgccactatt ttttttttga
     2281 aaattatgtt ttggcggaaa gttctttaaa aaagttaatc aaaatttttg gcgctttagt
     2341 caaattaatt ttctggcaaa aatattagta attttggcgt gagttcaatt ttcccattct
     2401 taaaatttca cacaattaag aattttaaga tttgggtttt tttttcaaca aaaaatcaaa
     2461 tatttacatg aaaattcggc gggaaataaa aaaaaatatt ccgattcaat gtgtgaataa
     2521 ctttttgcgg ggaattcaaa tttgcattat ttaaactttt tttaagctcg aaaaattgaa
     2581 attcttaaat aaataaataa aattttcctc gaattttgaa tgtttcgatt ttttagcgtt
     2641 attttttcaa aatttccact aaaactctag ttctaaagtt ctaaaacttt aaaaacttcc
     2701 taatgttgtg gtcttgtgtt tttttggcag cgatttccaa atttctgaaa ttccaacaaa
     2761 aaaaccacta cgtcagtttg catatccaac cccccccccc cccccccccc ctctgagcca
     2821 atcaatcatg tgcattttga attggcgcca aggcaatctt cgtaaaaaat aacaatagag
     2881 tcatccctca gaacagaaaa gaaacccgcc cgggggctca aatacacatg tcatttttct
     2941 tttttcgcct gatttttagc ctaaattagt gtttagcaat tttttaaggt aactcggtgg
     3001 agtcatttga actttttaaa ctaaaatttc agaaaaaatt cttttttttt gtcaaaaaat
     3061 aattttcccg ccggaatttt tcaaaaaaat aatttttatt ttatttttca agttccttgt
     3121 ttcctcttta tttttttatt ttgcccccaa aagttttaca ctaaaatatt taattttcag
     3181 gtaattcatc gaaagtcttt ctattttccg catctcttgt tcaaaacaag tttgtacaaa
     3241 aaagcaggct aaaaaatgcc aaagaagaag cgtaaggtcc cacaatttga catcctttgt
     3301 aaaaccccac caaaggtcct cgtccgtcaa ttcgtcgagc gtttcgagcg tccatccgga
     3361 gagaagatcg ccctctgcgc cgccgagctc acctacctct gctggatgat cacccacaac
     3421 ggaaccgcca tcaagcgtgc caccttcatg tcctacaaca ccatcatctc caactccctc
     3481 tccttcgaca tcgtcaacaa gtccctccaa ttcaagtaca agacccaaaa ggccaccatc
     3541 ctcgaggcct ccctcaagaa gctcatccca gcctgggagt tcaccatcat cccatactac
     3601 ggacaaaagc accaatccga catcaccgac atcgtctcct ccctccaact ccaattcgag
     3661 tcctccgagg aggccgacaa gggaaactcc cactccaaga aggtaagttt aaacatatat
     3721 atactaacta accctgatta tttaaatttt cagatgctca aggccctcct ctccgaggga
     3781 gagtccatct gggagatcac cgagaagatc ctcaactcct tcgagtacac ctcccgtttc
     3841 accaagacca agaccctcta ccaattcctc ttcctcgcca ccttcatcaa ctgcggacgt
     3901 ttctccgaca tcaagaacgt cgacccaaag tccttcaagc tcgtccaaaa caagtacctc
     3961 ggagtcatca tccaatgcct cgtcaccgag accaagacct ccgtctcccg tcacatctac
     4021 ttcttctccg cccgtggacg tatcgaccca ctcgtctacc tcgacgagtt cctccgtaac
     4081 tccgagccag tcctcaagcg tgtcaaccgt accggaaact cctcctccaa caagcaagag
     4141 taccaactcc tcaaggacaa cctcgtccgt tcctacaaca aggtaagttt aaacagttcg
     4201 gtactaacta accatacata tttaaatttt caggccctca agaagaacgc cccatactcc
     4261 atcttcgcca tcaagaacgg accaaagtcc cacatcggac gtcacctcat gacctccttc
     4321 ctctccatga agggactcac cgagctcacc aacgtcgtcg gaaactggtc cgacaagcgt
     4381 gcctccgccg tcgcccgtac cacctacacc caccaaatca ccgccatccc agaccactac
     4441 ttcgccctcg tctcccgtta ctacgcctac gacccaatct ccaaggagat gatcgccctc
     4501 aaggacgaga ccaacccaat cgaggagtgg caacacatcg agcaactcaa gggttccgcc
     4561 gagggatcca tccgttaccc agcctggaac ggaatcatct cccaagaggt cctcgactac
     4621 ctctcctcct acatcaaccg tcgtatctcc cgtcgtcgta aggccaaccc aaccaagctc
     4681 tccgagaacg ccaagaagct cgccaaggag gtcgagaact aaacccagct ttcttgtaca
     4741 aagtggggat gatcgacgcc aacgtcgttg aattttcaaa ttttaaatac tgaatatttg
     4801 ttttttttcc tattatttat ttattctctt tgtgtttttt ttcttgcttt ctaaaaaatt
     4861 aattcaatcc aaatctaaac attttttttt ctctttccgt ctcccaattc gtattccgct
     4921 cctctcatct gaacacaatg tgcaagttta tttatcttct cgctttcatt tcattaggac
     4981 gtggggggaa ttggtggaag ggggaaacac acaaaaggat gatggaaatg aaataaggac
     5041 acacaatatg caacaacatt caattcagaa atatggagga aggtttaaaa gaaaacataa
     5101 aaatatatag aggaggaagg aaaactagta aaaaataagc aaagaaatta ggcgaacgat
     5161 gagaattgtc ctcgcttgg
//
