LOCUS       sequence-136589-        3220 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP 
            by CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS338
FEATURES             Location/Qualifiers
     source          1..3220
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(130..158)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    717..750
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2714..2725
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2874..2907
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     3097..3117
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgggctccat tctcattcga aaaaatcctt gctgaagaac gtgaagctga ggagaatctc
       61 gcgggcagcg gtggcagtgg aggtaccggc ggaagcggta tgagcaaagg agaagaactt
      121 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      181 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      241 tgcactactg gaaaactacc tgttccatgg gtaagtttaa acatatatat actaactaac
      301 cctgattatt taaattttca gccaacactt gtcactactt tctgttatgg tgttcaatgc
      361 ttctcgagat acccagatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa
      421 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtaag
      481 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtgc tgaagtcaag
      541 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      601 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      661 gcagacaaac aaaagaatgg aatcaaagtt gtaagtttaa acatgatttt actaacataa
      721 cttcgtataa tgtatgctat acgaagttat ttctagacat tctctaatga aaaaatcttt
      781 cagttgaaat tgaaaatgag ttaaagttgg agtttttatt gaaaacagat ttccgtgtga
      841 ttagtgtttt tagcgagtgt gacaggacag cgaaaaaata tagaaacaag gggggaactg
      901 aaaagcttag gaatgcattg aacatgagaa ggggaagggg aaggaacaaa ctagacagga
      961 attattggaa tttaatcaca tttggagttt tttttctatt cgacagaata attatccaga
     1021 acatttttgt attaaatatt tatgcatcat atgagtagtc ggctttgttg tgcatgacga
     1081 gtttgttatc gacgaaatag aagctgtcag aacgagtctc gtttggattg ttgatcatgt
     1141 cgtccactga aaaagagatt agtcttttga attgtacttt ttagaataat gactcactga
     1201 gttgttgaga gagttgaggg aactcataga tatgttcaca gttgtttcgt gaattcggaa
     1261 tacagaatcc gaattcaaag tcaaaacact tcagaaggcg atctttgaag aagtgacgtt
     1321 cgatcattcg gaaatgatgg attgggatgt ctcctacttt gaattccaca gttgctccga
     1381 ccgttttgag tttcaaaaag tttggagcaa atctataacg cacgtatctt gccgactcct
     1441 gtggcgactc atcattctcc tgatcgttct ccggtttggc gatctcgaaa agcacttgct
     1501 cagtgtccag atcacggatt tggaacttgg tgaactcgat gttatagatg ttcgcagatg
     1561 gggagcataa gaatcctaaa tttatgtttt aaactgaaat ccaaagggag caagatacct
     1621 tgagtgattc ccggaagtgc taaaacgtcg ttcggagtga tttgagcttt cttcgcaagc
     1681 tccgattccg ttgtgattcc ttgttcggtg cttggtggtg gccgtggcat ctggaaatat
     1741 ggaaaagttc aacaaaaaga aaagagaaaa gaatgaaatc ggatatcaag agttagttga
     1801 gcggtttctc tagttttctg agtctcacct gcgacgggaa ggtcgccgag ccgggtggaa
     1861 tcgattgttg ttgctcggct ttcatatcgg tttggttgga agcggctgaa aacggaaaga
     1921 agtggaagaa ggaaaagagt gtggtgtgac aggaaaatgg taattagagg gtgccaaata
     1981 accagctata ttttgttttt ttttgaaaac atttttaaaa agaaaaatac gataatgata
     2041 tcagatggat ttccggaaaa ctggtatgaa aaatttcaac ctttttgagt acatgtaatc
     2101 aaaatacact ttgtaaatta tcatttttat tgaaactcca ccatttttct atttataacg
     2161 ctaataattt gaaaaagaaa cctgttgcga accgcggggt gaatcccaaa aacgaatgcg
     2221 ttttggtgga gtgattgatt cgaatcgaag aagaaaaaga agaagacgtg gaatagagag
     2281 ctcactctta accgagcagc acacaccgac agaaaaaaaa atgaaatgaa tgagggtctt
     2341 cttcttcttc ttcttcgaat gattgacaga aatgggaaaa agaggaagat tgagaaggga
     2401 aaaaggaagg agaaaagaag cagaagaaga cgtcagagag gagaggaacg agcggaaaag
     2461 cagcgggcgc aagtcataga agtagcagag ctggggagaa gaagacacta tccaagaaag
     2521 gaatgacgag agagtatgca aaggggtata gggtgcagac agaataggaa cagaataaca
     2581 gatgatgagc caagaagagt tgaaaagggc gatgaatttg tcatgtaact taatttgggt
     2641 caatttgagc atgatgaatt gaaatcatcc cttgttggga gttaataacc ggtttgttat
     2701 cagaaaccct gtaatagaag ggcgccctaa ctttgagcca attcatcccg gtttctgtca
     2761 aatatatcaa aaagtggtca actgacaaat tgtttttgat attataataa acattttatc
     2821 cgttaacaat tttcgaatac tttttacaag gacttggata aattggctca aagataactt
     2881 cgtataatgt atgctatacg aagttattaa ctaatctgat ttaaattttc agaacttcaa
     2941 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     3001 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     3061 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     3121 tgctgggatt acacatggca tggatgaact atacaaaggt taagatcacc tcggccactt
     3181 caaacagtgt gacatcgacg ttcgacaaat ctttaatgta
//
