LOCUS       sequence-136601-        3203 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS292
FEATURES             Location/Qualifiers
     source          1..3203
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..38
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    725..758
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2722..2733
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2882..2915
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gctcttcgat gactgctcca aagaagaagc gtaaggtacc ggtagaaaaa gggatggtct
       61 caaagggtga agaagataac atggcaatta ttaaagagtt tatgcgtttc aaggtgcata
      121 tggagggatc tgtcaatggg catgagtttg aaattgaagg tgaaggagaa ggccgaccat
      181 atgagggaac acaaaccgca aaactaaagg taagtttaaa catatatata ctaactaacc
      241 ctgattattt aaattttcag gtaactaaag gcggaccatt accattcgcc tgggacatcc
      301 tctctccaca gttcatgtat ggaagtaaag cttatgttaa acatccggca gatataccag
      361 attatttgaa actttcattc ccggagggtt ttaagtggga acgcgtaatg aattttgaag
      421 acggaggagt tgttacagtg acgcaagact caaggtaagt ttaaacagtt cggtactaac
      481 taaccataca tatttaaatt ttcagcctcc aagatggaga atttatttat aaagtcaaac
      541 ttcgaggaac gaatttcccc tcggatggac ctgttatgca gaagaagact atgggatggg
      601 aagcttcaag tgaaagaatg taccctgaag acggtgctct taagggagag attaaacaac
      661 gtcttaaatt gaaagatgga ggacattacg atgctgaggt aagtttaaac atgattttac
      721 taacataact tcgtataatg tatgctatac gaagttattt ctagacattc tctaatgaaa
      781 aaatctttca gttgaaattg aaaatgagtt aaagttggag tttttattga aaacagattt
      841 ccgtgtgatt agtgttttta gcgagtgtga caggacagcg aaaaaatata gaaacaaggg
      901 gggaactgaa aagcttagga atgcattgaa catgagaagg ggaaggggaa ggaacaaact
      961 agacaggaat tattggaatt taatcacatt tggagttttt tttctattcg acagaataat
     1021 tatccagaac atttttgtat taaatattta tgcatcatat gagtagtcgg ctttgttgtg
     1081 catgacgagt ttgttatcga cgaaatagaa gctgtcagaa cgagtctcgt ttggattgtt
     1141 gatcatgtcg tccactgaaa aagagattag tcttttgaat tgtacttttt agaataatga
     1201 ctcactgagt tgttgagaga gttgagggaa ctcatagata tgttcacagt tgtttcgtga
     1261 attcggaata cagaatccga attcaaagtc aaaacacttc agaaggcgat ctttgaagaa
     1321 gtgacgttcg atcattcgga aatgatggat tgggatgtct cctactttga attccacagt
     1381 tgctccgacc gttttgagtt tcaaaaagtt tggagcaaat ctataacgca cgtatcttgc
     1441 cgactcctgt ggcgactcat cattctcctg atcgttctcc ggtttggcga tctcgaaaag
     1501 cacttgctca gtgtccagat cacggatttg gaacttggtg aactcgatgt tatagatgtt
     1561 cgcagatggg gagcataaga atcctaaatt tatgttttaa actgaaatcc aaagggagca
     1621 agataccttg agtgattccc ggaagtgcta aaacgtcgtt cggagtgatt tgagctttct
     1681 tcgcaagctc cgattccgtt gtgattcctt gttcggtgct tggtggtggc cgtggcatct
     1741 ggaaatatgg aaaagttcaa caaaaagaaa agagaaaaga atgaaatcgg atatcaagag
     1801 ttagttgagc ggtttctcta gttttctgag tctcacctgc gacgggaagg tcgccgagcc
     1861 gggtggaatc gattgttgtt gctcggcttt catatcggtt tggttggaag cggctgaaaa
     1921 cggaaagaag tggaagaagg aaaagagtgt ggtgtgacag gaaaatggta attagagggt
     1981 gccaaataac cagctatatt ttgttttttt ttgaaaacat ttttaaaaag aaaaatacga
     2041 taatgatatc agatggattt ccggaaaact ggtatgaaaa atttcaacct ttttgagtac
     2101 atgtaatcaa aatacacttt gtaaattatc atttttattg aaactccacc atttttctat
     2161 ttataacgct aataatttga aaaagaaacc tgttgcgaac cgcggggtga atcccaaaaa
     2221 cgaatgcgtt ttggtggagt gattgattcg aatcgaagaa gaaaaagaag aagacgtgga
     2281 atagagagct cactcttaac cgagcagcac acaccgacag aaaaaaaaat gaaatgaatg
     2341 agggtcttct tcttcttctt cttcgaatga ttgacagaaa tgggaaaaag aggaagattg
     2401 agaagggaaa aaggaaggag aaaagaagca gaagaagacg tcagagagga gaggaacgag
     2461 cggaaaagca gcgggcgcaa gtcatagaag tagcagagct ggggagaaga agacactatc
     2521 caagaaagga atgacgagag agtatgcaaa ggggtatagg gtgcagacag aataggaaca
     2581 gaataacaga tgatgagcca agaagagttg aaaagggcga tgaatttgtc atgtaactta
     2641 atttgggtca atttgagcat gatgaattga aatcatccct tgttgggagt taataaccgg
     2701 tttgttatca gaaaccctgt aatagaaggg cgccctaact ttgagccaat tcatcccggt
     2761 ttctgtcaaa tatatcaaaa agtggtcaac tgacaaattg tttttgatat tataataaac
     2821 attttatccg ttaacaattt tcgaatactt tttacaagga cttggataaa ttggctcaaa
     2881 gataacttcg tataatgtat gctatacgaa gttattaact aatctgattt aaattttcag
     2941 gtgaagacaa cttacaaagc caaaaaacca gttcagctgc caggagcgta caatgttaat
     3001 attaaactgg atatcacctc ccacaacgag gattacacta tcgttgagca atatgaaaga
     3061 gctgaagggc ggcactcgac aggtggcatg gatgaattgt ataaggggag ctctatgagc
     3121 cgtagacgaa aagcgaatcc gacaaaactg agtgaaaacg cgaagaagct tgccaaggaa
     3181 gttgaaaatt aaggtcgaag agc
//
