LOCUS       sequence-136629-        1467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pWD509
FEATURES             Location/Qualifiers
     source          1..1467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..24
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1393..1464
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
ORIGIN
        1 atgccaaaga agaagcgtaa ggtcccacaa tttgacatcc tttgtaaaac cccaccaaag
       61 gtcctcgtcc gtcaattcgt cgagcgtttc gagcgtccat ccggagagaa gatcgccctc
      121 tgcgccgccg agctcaccta cctctgctgg atgatcaccc acaacggaac cgccatcaag
      181 cgtgccacct tcatgtccta caacaccatc atctccaact ccctctcctt cgacatcgtc
      241 aacaagtccc tccaattcaa gtacaagacc caaaaggcca ccatcctcga ggcctccctc
      301 aagaagctca tcccagcctg ggagttcacc atcatcccat actacggaca aaagcaccaa
      361 tccgacatca ccgacatcgt ctcctccctc caactccaat tcgagtcctc cgaggaggcc
      421 gacaagggaa actcccactc caagaaggta agtttaaaca tatatatact aactaaccct
      481 gattatttaa attttcagat gctcaaggcc ctcctctccg agggagagtc catctgggag
      541 atcaccgaga agatcctcaa ctccttcgag tacacctccc gtttcaccaa gaccaagacc
      601 ctctaccaat tcctcttcct cgccaccttc atcaactgcg gacgtttctc cgacatcaag
      661 aacgtcgacc caaagtcctt caagctcgtc caaaacaagt acctcggagt catcatccaa
      721 tgcctcgtca ccgagaccaa gacctccgtc tcccgtcaca tctacttctt ctccgcccgt
      781 ggacgtatcg acccactcgt ctacctcgac gagttcctcc gtaactccga gccagtcctc
      841 aagcgtgtca accgtaccgg aaactcctcc tccaacaagc aagagtacca actcctcaag
      901 gacaacctcg tccgttccta caacaaggta agtttaaaca gttcggtact aactaaccat
      961 acatatttaa attttcaggc cctcaagaag aacgccccat actccatctt cgccatcaag
     1021 aacggaccaa agtcccacat cggacgtcac ctcatgacct ccttcctctc catgaaggga
     1081 ctcaccgagc tcaccaacgt cgtcggaaac tggtccgaca agcgtgcctc cgccgtcgcc
     1141 cgtaccacct acacccacca aatcaccgcc atcccagacc actacttcgc cctcgtctcc
     1201 cgttactacg cctacgaccc aatctccaag gagatgatcg ccctcaagga cgagaccaac
     1261 ccaatcgagg agtggcaaca catcgagcaa ctcaagggtt ccgccgaggg atccatccgt
     1321 tacccagcct ggaacggaat catctcccaa gaggtcctcg actacctctc ctcctacatc
     1381 aaccgtcgta tctcccgtcg tcgtaaggcc aacccaacca agctctccga gaacgccaag
     1441 aagctcgcca aggaggtcga gaactaa
//
