LOCUS       sequence-136631-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BrdU-incorporating plasmid with selectable marker HIS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Viggiani CJ, Aparicio OM
  TITLE     New vectors for simplified construction of BrdU-Incorporating 
            strains of Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Oct-Nov;23(14-15):1045-51.
  PUBMED    17083135
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Oct-Nov"; volume: "23(14-15)"; pages: "1045-51"
COMMENT     Sequence Label: p403-BrdU-Inc
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        133..320
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             321..983
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 taaattcccg ttttaagagc ttggtgagcg ctaggagtca ctgccaggta tcgtttgaac
       61 acggcattag tcagggaagt cataacacag tcctttcccg caattttctt tttctattac
      121 tcttggcctc ctctagtaca ctctatattt ttttatgcct cggtaatgat tttcattttt
      181 ttttttccac ctagcggatg actctttttt tttcttagcg attggcatta tcacataatg
      241 aattatacat tatataaagt aatgtgattt cttcgaagaa tatactaaaa aatgagcagg
      301 caagataaac gaaggcaaag atgacagagc agaaagccct agtaaagcgt attacaaatg
      361 aaaccaagat tcagattgcg atctctttaa agggtggtcc cctagcgata gagcactcga
      421 tcttcccaga aaaagaggca gaagcagtag cagaacaggc cacacaatcg caagtgatta
      481 acgtccacac aggtataggg tttctggacc atatgataca tgctctggcc aagcattccg
      541 gctggtcgct aatcgttgag tgcattggtg acttacacat agacgaccat cacaccactg
      601 aagactgcgg gattgctctc ggtcaagctt ttaaagaggc cctaggggcc gtgcgtggag
      661 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
      721 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
      781 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
      841 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
      901 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
      961 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
     1021 atacatgtgt atatatgtat acc
//
