LOCUS       sequence-136849-        4373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Peredox fluorescent sensor protein (for NADH/NAD 
            ratio) in bacteria, with N-terminal his-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Mongeon R, Venkatachalam V, Yellen G
  TITLE     Cytosolic NADH-NAD Redox Visualized in Brain Slices by Two-Photon 
            Fluorescence Lifetime Biosensor Imaging.
  JOURNAL   Antioxid Redox Signal. 2016 Mar 18.
  PUBMED    26857245
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antioxid 
            Redox Signal. 2016 Mar 18."
COMMENT     Sequence Label: pRSetB-Peredox
FEATURES             Location/Qualifiers
     source          1..4373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     138..155
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     240..259
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        240..258
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             290..312
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             326..346
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     2449..2470
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2633..2737
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2738..2806,2807..3598)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2956..2975)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3769..4357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4258..4277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
       61 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      121 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
      181 aaccgcctct ccccgcgcgt tggccgattc attaatgcag gatctcgatc ccgcgaaatt
      241 aatacgactc actataggga gaccacaacg gtttccctct agaaataatt ttgtttaact
      301 ttaagaagga gatatacata tgaaacatca ccatcatcat catcatggtg gagctagcat
      361 gaaagttcct gaagcagcca tttccagact gattacttac ctgagaatcc tggaagagct
      421 ggaagcccaa ggtgttcacc gcaccgcctc cgagcaactg ggcgagctgg cccaggtcac
      481 cgccttccag gttgataagg acctgtccta cttcggctcc tacggcactg acggcgttgg
      541 ctacactgta ccggtcctca agagagaact gcgccatatc ctcggtctga accgcaaatg
      601 gggcctgtgc atcgtgggga tgggccgcct gggatccgct cttgctgatt ggcctggttt
      661 tggcgagagc ttcgagctgc gcggtttctt cgatgttgac ccaggtatgg ttggtcggcc
      721 ggttcgcggt ggtgttatcg aacacgttga tctgctgccc cagcgcgtac ctggtcgcat
      781 cgaaatcgct ctgctgacgg ttccacgcga ggcagcacag aaggctgccg acctgctggt
      841 tgccgctggc atcaagggca tcctgaactt cgctccagtt gtactggagg ttcccaagga
      901 agtggctgtt gagaacgttg acatcctggc cggtctgacg cgtctgagct ttgccattct
      961 gaaccccacg tggagcgcag caggtgggca tggtttcact gctcacaacg tgtatatcat
     1021 ggctgacaaa cagaagaacg gtatcaaggc caacttcaag atccgccaca acatcgaaga
     1081 cggcggcgtt cagctggctg atcactacca gcagaacacc ccaatcggcg acggccctgt
     1141 tctgctgcct gacaaccact acttaagcat ccagtctaaa ctgtccaaag acccaaacga
     1201 gaagcgcgac cacatggttc tgctggaatt tgttaccgcc gctggcatca cccacggcat
     1261 ggacgaactg tacaagggcg gcaccggggg atctatggtt tccaaaggcg aggaactgtt
     1321 cactggtgtt gttcctatcc tggttgaact ggacggcgac gttaacggcc acaaattcag
     1381 cgttagcggg gaaggcgaag gagacgctac ctacggcaag ctgactctga agttcatctg
     1441 caccactggc aagctgcctg ttccttggcc taccctggtt actaccttct cctatggtgt
     1501 tatggttttc gccagatatc ctgaccacat gaagcagcac gacttcttca agagcgccat
     1561 gcctgagggc tacgttcagg aacgcaccat ctttttcaag gacgacggca actataagac
     1621 acgcgctgag gttaagttcg agggcgacac tctggttaac cgcatcgagc tgaagggcat
     1681 cgacttcaag gaggacggca acatcctggg ccataagctt gaatataaca ccaaggtacc
     1741 tgaagccgcc atcagccgcc tcatcactta cctccgcatc ctggaggagc tcgaagccca
     1801 aggcgttcac cgcactgcaa gcgagcagct gggcgaactg gcacaggtta cagcattcca
     1861 ggttgacgaa gatctgtcct atttcggttc ctacggcacc gacggtgttg gctacacagt
     1921 ccctgtcctg aagcgcgagc tgcgccacat cctgggcctg aaccgcaagt ggggcttatg
     1981 catcgttggc atgggccgcc tcggtagtgc actggcagac tggccgggct ttggtgaatc
     2041 tttcgaactg cgcggcttct tcgacgttga tccaggcatg gttgggcgcc ctgttcgcgg
     2101 tggcgttatc gagcatgttg acctcctgcc acagcgcgtc ccgggtcgca tcgagatcgc
     2161 cctgctgacc gttcctcgcg aagctgcaca gaaggcagcc gacttactag tcgccgctgg
     2221 tatcaaggga attctgaact tcgcccctgt tgttctggag gttcctaagg aagttgctgt
     2281 tgaaaacgtt gatttcctgg ccggcctgac ccgcctgagc ttcgcaatcc tgaatcctaa
     2341 gtggcgcgag gaaatgatgg gttgactcga ggggcccaag ctgtagtggg ccatcgccct
     2401 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2461 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     2521 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     2581 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     2641 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2701 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     2761 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2821 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     2881 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     2941 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     3001 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3061 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3121 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3181 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     3241 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     3301 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3361 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3421 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     3481 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     3541 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3601 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     3661 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3721 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3781 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3841 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3901 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3961 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4021 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4081 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4141 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4201 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4261 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4321 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cgg
//
