LOCUS       sequence-137168-        5046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Conklin BR, Farfel Z, Lustig KD, Julius D, Bourne HR
  TITLE     Substitution of three amino acids switches receptor specificity of 
            Gq alpha to that of Gi alpha.
  JOURNAL   Nature. 1993 May 20. 363(6426):274-6.
  PUBMED    8387644
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1993 May 20. 363(6426):274-6."
COMMENT     Sequence Label: qz5
FEATURES             Location/Qualifiers
     source          1..5046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(19..477)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(156..177)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     371..390
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     tRNA            complement(join(600..619,620..704,705..779))
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     enhancer        952..1331
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1332..1535
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1486..1506
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1580..1599
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1580..1598
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2729..2747)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2730..2747)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          2831..2896
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3026..3046
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3318..3452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3355..3374)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3409..3428
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4303..4438
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4933..4952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagctagctt ctagctagaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt
       61 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
      121 caaaagaata gcccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
      181 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggccgcc
      241 cactacgtga accatcaccc aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa
      301 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
      361 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
      421 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtact
      481 atggttgctt tgacgagcac gtataacgtg ctttcctcgt tagaatcaga gcgggagcta
      541 aacaggaggc cgattaaagg gattttagac aggaacggta cgccagctgg accgcggtct
      601 ttcggacttt tgaaagtgat ggtggtgggg gaaggattcg aaccttcgaa gtcgatgacg
      661 gcagatttag agtctgctcc ctttggccgc tcgggaaccc caccacaggt aatgctttta
      721 ctggcctgct cccttatcgg gaagcggggc gcatcatatc aaatgacgcg ccgctgtaaa
      781 gtgttacgtt gagaaagctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
      841 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
      901 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
      961 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     1021 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     1081 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     1141 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     1201 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     1261 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     1321 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     1381 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     1441 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     1501 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
     1561 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag
     1621 ctcggatcca tgactctgga gtccatcatg gcatgctgcc tgagcgagga ggccaaggaa
     1681 gcccggagga tcaacgacga gatcgagcgg cagctgcgca gggacaagcg cgacgcccgc
     1741 cgggagctca agctgctgct gctggggaca ggggagagtg gcaagtcgac cttcatcaag
     1801 cagatgagga tcatccacgg gtcgggctac tctgacgaag acaagcgcgg cttcaccaag
     1861 ctggtgtatc agaacatctt cacggccatg caggccatga tcagagcgat ggacacactc
     1921 aagatcccat acaagtatga acacaataag gctcatgcac aattggttcg agaggttgat
     1981 gtggagaagg tgtctgcttt tgacgtcccc gactacgcgg caataaagag cttgtggaat
     2041 gatcctggaa tccaggagtg ctacgacaga cgacgggaat atcagttatc tgactctacc
     2101 aaatactatc tgaatgactt ggaccgtgta gccgaccctt cctatctgcc tacacaacaa
     2161 gacgtgctta gagttcgagt ccccactaca gggatcatcg aatacccctt tgacttacaa
     2221 agtgtcattt tcagaatggt cgatgtaggg ggccaaaggt cagagagaag aaaatggata
     2281 cactgctttg aaaatgtcac ctccatcatg tttctagtag cgcttagcga atatgatcaa
     2341 gttcttgtgg agtcagacaa tgagaaccgc atggaggaga gcaaagcact ctttagaaca
     2401 attatcacct acccctggtt ccagaactcc tctgtgattc tgttcttaaa caagaaagat
     2461 cttctagagg agaaaatcat gtattcccac ctagtcgact acttcccaga atatgatgga
     2521 ccccagagag atgcccaggc agctcgagaa ttcatcctga aaatgttcgt ggacctgaac
     2581 cccgacagtg acaaaatcat ctactcccac ttcacgtgcg ccacagatac cgagaacatc
     2641 cgcttcgtct ttgcagccgt caaggacacc atcctgcagc tgaacctgaa gtacattggc
     2701 ctttgctaat gcatctagag ggccctattc tatagtgtca cctaaatgct agaggatctt
     2761 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     2821 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     2881 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     2941 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     3001 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     3061 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     3121 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     3181 aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     3241 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     3301 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3361 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3421 aactcatcaa tgtatcttat catgtctgga tcgatcccgc catggtatca acgccatatt
     3481 tctatttaca gtagggacct cttcgttgtg taggtaccgc tgtattccta gggaaatagt
     3541 agaggcacct tgaactgtct gcatcagcca tatagccccc gctgttcgat ttacaaacac
     3601 aggcacagta ctgacaaacc catacacctc ctctgaaata cccatagttg ctagggctgt
     3661 ctccgaactc attacaccct ccaaagtcag agctgtaatt tcgccatcaa gggcagcgag
     3721 ggcttctcca gataaaatag cttctgccga gagtcccgta agggtagaca cttcagctaa
     3781 tccctcgatg aggtctacta gaatagtcag tgcggctccc attttgaaaa ttcacttact
     3841 tgatcagctt cagaagatgg cggagggcct ccaacacagt aattttcctc ccgactctta
     3901 aaatagaaaa tgtcaagtca gttaagcagg aagtgactaa ctgaccgcag ctggccgtgc
     3961 gacatcctct tttaattagt tgctaggcaa ctgccctcca gagggcagtg tggttttgca
     4021 agaggaagca aaaagcctct ccacccaggc ctagaatgtt tccacccaat cattactatg
     4081 acaacagctg ttttttttag tattaagcag aggccggggg cccctggcct ccgcttactc
     4141 tggagaaaaa gaagagaggc attgtagagg cttccagagg caacttgtca aaacaggact
     4201 gcttctattt ctgtcacact gtctggccct gtcacaaggt ccagcacctc cataccccct
     4261 ttaataagca gtttgggaac gggtgcgggt cttactccgc ccatcccgcc cctaactccg
     4321 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4381 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4441 ggcttttgca aaaagctaat tcggcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4501 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4561 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     4621 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctgaagccag ttaccagtgg
     4681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagagat agttaccgga
     4741 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4801 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4861 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4921 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4981 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5041 caacgc
//
