LOCUS       sequence-137212-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RHOA
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(656..676)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(701..718)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             729..751
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    766..790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(790..809)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(791..809)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcacccacgt ctagcttgca
      121 gagcagctct cgtagccatt tcaaaaacct ctctcactcc atctttggtc tttgctgaac
      181 actccatgta cccaaaagcg ccaatcctgt ttgccatatc tctgccttct tcaggtttca
      241 ccggctcctg cttcatcttg gctagctccc gccttgtgtg ctcatcattc cgaagatcct
      301 tcttattccc aaccaggatg atgggcacgt tgggacagaa atgcttgact tctggggtcc
      361 acttttctgg gatgttttct aaactatcag ggctgtcgat ggaaaaacac atcagtataa
      421 catcggtatc tgggtaggag aggggcctca ggcgatcata atcttcctgc ccagctgtgt
      481 cccacaaagc caattctacc tgctttccat ccacctcgat atctgccaca tagttctcaa
      541 acactgtggg cacatacacc tctgggaact ggtccttgct gaagactatg agcaagcatg
      601 tctttccaca ggctccatca ccaacaatca ccagtttctt ccggatggca gccatggatt
      661 ggaagtacag gttctcggta cccagatcta caccagaaga atgatgatga tgatggtgca
      721 tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc
      781 tcacaattcc cctatagtga gtcgtattaa
//
