LOCUS       sequence-137244-       10081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: tetO-TBX5
FEATURES             Location/Qualifiers
     source          1..10081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(993..1784,1785..1853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1616..1635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1854..1958)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2033..2052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2224..2603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2605..2803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2821..3001
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3048..3173
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3666..3899
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4084..4128
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4426..4543
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4596..4614
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4638..4656
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4680..4698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4722..4740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4764..4782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4815..4853
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4850..4874
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     4953..4972
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4953..4971
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6591..6615)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6641..6682
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6692..6709
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6759..7347
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6812..6832)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7230..7241)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7350..7366)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7351..7367)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7872..8052
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8078..8095)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8084..8308
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8354..8782
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8441..8460)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8651..8672
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8791..8811)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8796..9125
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8976..9111
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9038..9057
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9173..9220
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9239..9613
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9743..9864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9780..9799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9834..9853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9913..9929)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9913..9929)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9926..9948)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9937..9953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9961..9967,9968..9985,9986..9991))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10006..10027
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1981 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     2041 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     2101 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     2161 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     2221 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     2281 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2341 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2401 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2461 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2521 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2581 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2641 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2701 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2761 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
     2821 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2881 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2941 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3001 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3061 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3121 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     3181 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     3241 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     3301 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     3361 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     3421 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     3481 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     3541 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3601 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     3661 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     3721 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     3781 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     3841 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     3901 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3961 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4021 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4081 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4141 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     4201 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     4261 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     4321 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     4381 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     4441 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     4501 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     4561 cagcagagat ccagtttggt tatcgagttt accactccct atcagtgata gagaaaagtg
     4621 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     4681 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     4741 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     4801 ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     4861 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     4921 gggaccgatc cagcctccgc ggccccgaat tgtaatacga ctcactatag ggagacccaa
     4981 gctggctagg taagcttgat caacaagttt gtacaaaaaa gcaggctcca ccatggccga
     5041 cgcagacgag ggctttggcc tggcgcacac gcctctggag cctgacgcaa aagacctgcc
     5101 ctgcgattcg aaacccgaga gcgcgctcgg ggcccccagc aagtccccgt cgtccccgca
     5161 ggccgccttc acccagcagg gcatggaggg aatcaaagtg tttctccatg aaagagaact
     5221 gtggctaaaa ttccacgaag tgggcacgga aatgatcata accaaggctg gaaggcggat
     5281 gtttcccagt tacaaagtga aggtgacggg ccttaatccc aaaacgaagt acattcttct
     5341 catggacatt gtacctgccg acgatcacag atacaaattc gcagataata aatggtctgt
     5401 gacgggcaaa gctgagcccg ccatgcctgg ccgcctgtac gtgcacccag actcccccgc
     5461 caccggggcg cattggatga ggcagctcgt ctccttccag aaactcaagc tcaccaacaa
     5521 ccacctggac ccatttgggc atattattct aaattccatg cacaaatacc agcctagatt
     5581 acacatcgtg aaagcggatg aaaataatgg atttggctca aaaaatacag cgttctgcac
     5641 tcacgtcttt cctgagactg cgtttatagc agtgacttcc taccagaacc acaagatcac
     5701 gcaattaaag attgagaata atccctttgc caaaggattt cggggcagtg atgacatgga
     5761 gctgcacaga atgtcaagaa tgcaaagtaa agaatatccc gtggtcccca ggagcaccgt
     5821 gaggcaaaaa gtggcctcca accacagtcc tttcagcagc gagtctcgag ctctctccac
     5881 ctcatccaat ttggggtccc aataccagtg tgagaatggt gtttccggcc cctcccagga
     5941 cctcctgcct ccacccaacc catacccact gccccaggag catagccaaa tttaccattg
     6001 taccaagagg aaagaggaag aatgttccac cacagaccat ccctataaga agccctacat
     6061 ggagacatca cccagtgaag aagattcctt ctaccgctct agctatccac agcagcaggg
     6121 cctgggtgcc tcctacagga cagagtcggc acagcggcaa gcttgcatgt atgccagctc
     6181 tgcgcccccc agcgagcctg tgcccagcct agaggacatc agctgcaaca cgtggccaag
     6241 catgccttcc tacagcagct gcaccgtcac caccgtgcag cccatggaca ggctacccta
     6301 ccagcacttc tccgctcact tcacctcggg gcccctggtc cctcggctgg ctggcatggc
     6361 caaccatggc tccccacagc tgggagaggg aatgttccag caccagacct ccgtggccca
     6421 ccagcctgtg gtcaggcagt gtgggcctca gactggcctg cagtcccctg gcacccttca
     6481 gccccctgag ttcctctact ctcatggcgt gccaaggact ctatcccctc atcagtacca
     6541 ctctgtgcac ggagttggca tggtgccaga gtggagcgac aatagcttgg acccagcttt
     6601 cttgtacaaa gtggttgatc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
     6661 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac
     6721 ccgctgatca gcctcaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     6781 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6841 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6901 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6961 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7021 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7081 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7141 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     7201 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7261 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7321 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     7381 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     7441 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     7501 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7561 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     7621 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     7681 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     7741 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7801 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     7861 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7921 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7981 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8041 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     8101 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     8161 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     8221 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     8281 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     8341 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8401 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8461 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8521 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8581 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8641 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8701 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8761 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8821 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8881 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8941 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     9001 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9061 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9121 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     9181 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     9241 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     9301 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     9361 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     9421 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     9481 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     9541 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     9601 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     9661 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9721 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     9781 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9841 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     9901 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9961 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    10021 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    10081 g
//
