LOCUS       sequence-137299-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing FKBPF36V protein in washing out shield-1 ligand for 
            FKBPdd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Egeler EL, Urner LM, Rakhit R, Liu CW, Wandless TJ
  TITLE     Ligand-switchable substrates for a ubiquitin-proteasome system.
  JOURNAL   J Biol Chem. 2011 Sep 9;286(36):31328-36. doi: 
            10.1074/jbc.M111.264101. Epub 2011 Jul 15.
  PUBMED    21768107
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.264101"; journalName: "J Biol Chem"; date: 
            "2011-09-9- 9"; volume: "286"; issue: "36"; pages: "31328-36"
COMMENT     Sequence Label: pET15b His6-FKBPF36V
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..72
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(112..114,115..438)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     primer_bind     complement(489..507)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      503..550
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag cggcctggtg
       61 ccgcgcggca gccatatgga aaacctgtat tttcagggag gtggaggatc catgggagtg
      121 caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc
      181 gtggtgcact acaccgggat gcttgaagat ggaaagaaag ttgattcctc ccgggacaga
      241 aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg
      301 gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat
      361 ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag
      421 cttctaaaac tggaatgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      481 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      541 gggttttttg ctgaaaggag gaactatatc cggatatccc gcaagaggcc cggcagtacc
      601 ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc
      661 gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta
      721 ccgcat
//
