LOCUS       sequence-137349-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA against CRTC2 (3rd generation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Jeanneteau FD, Lambert WM, Ismaili N, Bath KG, Lee FS, Garabedian 
            MJ, Chao MV
  TITLE     BDNF and glucocorticoids regulate corticotrophin-releasing hormone 
            (CRH) homeostasis in the hypothalamus.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1305-10. doi: 
            10.1073/pnas.1114122109. Epub 2012 Jan 9.
  PUBMED    22232675
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1114122109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-01-24- 24"; volume: "109"; issue: "4"; pages: "1305-10"
COMMENT     Sequence Label: pLL3.7 shRNA against CRTC2
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..53
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     38..54
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(96..129)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        245..548
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        549..752
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     703..723
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      791..800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagatgttga attgctccag attccttttt gaattctcga ggtcgacggt atcgataagc
       61 tcgcttcacg agattccagc aggtcgaggg acctaataac ttcgtatagc atacattata
      121 cgaagttata ttaagggttc caagcttaag cggccgcgtg gataaccgta ttaccgccat
      181 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      241 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      301 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      361 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      421 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      481 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      541 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      601 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      661 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      721 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
      781 gctaccggtc gccaccatgg
//
