LOCUS       sequence-137443-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector for tet-inducible expression of EGFP 
            tagged human RHOA-T19N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Kong YP, Carrion B, Singh RK, Putnam AJ
  TITLE     Matrix identity and tractional forces influence indirect cardiac 
            reprogramming.
  JOURNAL   Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474.
  PUBMED    24326998
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-12-11"; volume: "3:3474. doi"; pages: " 
            10.1038/srep03474"
COMMENT     Sequence Label: tetO-FUW-eGFP-RHOA-T19N
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(53..577,578..580,581..634))
                     /codon_start=1
                     /product="dominant negative mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (T19N)
                     /translation="MAAIRKKLVIVGDGACGKNCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     primer_bind     complement(683..704)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcggat cctcacaaga
       61 caaggcaccc agattttttc ttcccacgtc tagcttgcag agcagctctc gtagccattt
      121 caaaaacctc tctcactcca tctttggtct ttgctgaaca ctccatgtac ccaaaagcgc
      181 caatcctgtt tgccatatct ctgccttctt caggtttcac cggctcctgc ttcatcttgg
      241 ctagctcccg ccttgtgtgc tcatcattcc gaagatcctt cttattccca accaggatga
      301 tgggcacgtt gggacagaaa tgcttgactt ctggggtcca cttttctggg atgttttcta
      361 aactatcagg gctgtcgatg gaaaaacaca tcagtataac atcggtatct gggtaggaga
      421 ggggcctcag gcgatcataa tcttcctgcc cagctgtgtc ccacaaagcc aactctacct
      481 gctttccatc cacctcgata tctgccacat agttctcaaa cactgtgggc acatacacct
      541 ctgggaactg gtccttgctg aagactatga gcaagcagtt ctttccacag gctccatcac
      601 caacaatcac cagtttcttc cggatggcag ccatgaattc cttgtacagc tcgtccatgc
      661 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      721 tggggtcttt gctcagggcg gactg
//
