LOCUS       sequence-137614-       10171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BrdU-incorporating plasmid with selectable marker TRP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Viggiani CJ, Aparicio OM
  TITLE     New vectors for simplified construction of BrdU-Incorporating 
            strains of Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Oct-Nov;23(14-15):1045-51.
  PUBMED    17083135
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Oct-Nov"; volume: "23(14-15)"; pages: "1045-51"
COMMENT     Sequence Label: p404-BrdU-Inc
FEATURES             Location/Qualifiers
     source          1..10171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        187..468
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             469..1143
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1246..1701)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1383..1404)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1595..1614
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1831..1853
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1845..1862
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1846..1862
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1872..1891
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1872..1890
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2937..2985
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3010..3029
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3027..4157)
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLERK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHVGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWWEDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     promoter        complement(4176..4819)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(4260..4283)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     promoter        5725..6428
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     complement(7911..7931)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7911..7929)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7950..7966)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7950..7966)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7963..7985)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7974..7990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7998..8004,8005..8022,8023..8028))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8181..8198)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8352..8940)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8432..8451)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9111..9902,9903..9971))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9734..9753
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9972..10076)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10144..10162
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaac agcatcgtaa
      241 tatatgtgta ctttgcagtt atgacgccag atggcagtag tggaagatat tctttattga
      301 aaaatagctt gtcaccttac gtacaatctt gatccggagc ttttcttttt ttgccgatta
      361 agaattaatt cggtcgaaaa aagaaaagga gagggccaag agggagggca ttggtgacta
      421 ttgagcacgt gagtatacgt gattaagcac acaaaggcag cttggagtat gtctgttatt
      481 aatttcacag gtagttctgg tccattggtg aaagtttgcg gcttgcagag cacagaggcc
      541 gcagaatgtg ctctagattc cgatgctgac ttgctgggta ttatatgtgt gcccaataga
      601 aagagaacaa ttgacccggt tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa
      661 aatagttcag gcactccgaa atacttggtt ggcgtgtttc gtaatcaacc taaggaggat
      721 gttttggctc tggtcaatga ttacggcatt gatatcgtcc aactgcatgg agatgagtcg
      781 tggcaagaat accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa
      841 gactgcaaca tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt
      901 gattcagaag caggtgggac aggtgaactt ttggattgga actcgatttc tgactgggtt
      961 ggaaggcaag agagccccga aagcttacat tttatgttag ctggtggact gacgccagaa
     1021 aatgttggtg atgcgcttag attaaatggc gttattggtg ttgatgtaag cggaggtgtg
     1081 gagacaaatg gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa
     1141 taggttatta ctgagtagta tttatttaag tattgtttgt gcacttgccg atctatgcgg
     1201 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     1261 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     1321 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     1381 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     1441 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     1501 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     1561 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     1621 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     1681 atgcgccgct acagggcgcg tcgcgccatt cgccattcag gctgcgcaac tgttgggaag
     1741 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     1801 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     1861 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg tacccgggga tctagtggat
     1921 ccgcgaattc ccgcccgtag cgcgcgcgca ccagcgcggc atcgcacccg agcaccagct
     1981 cccccgtcgt ccagatgccc acgggccacg tcgaggccga cggggagaaa tacacgtacc
     2041 tacctgggga tctcaacagg ccccgggtgg ccaaccaggt cgtggacgcg ttgtgcaggt
     2101 gcgtgatgtc cagctccgtc gtcgggtgcc gccgggcccc aaccggcggt cgggggggcg
     2161 gtgtatcacg cggcccgctc gggtggctcg ccgtcgccac gttgtctccc cgcgggaacg
     2221 tcagggcctc ggggtcaggg acggccgaaa acgttaccca ggcccgggaa cgcagcaaca
     2281 cggaggcggt tggattgtgc aagagaccct taaggggggc gaccgcgggg ggaggctggg
     2341 cggtcggctc gaccgtgatg ggggcgggca ggctcgcgtt cgggggccgg ccgagtaggt
     2401 aggtcttcga gatgtaaagc agctggccgg ggtcccgcgg aaactcggcc gtggtgacca
     2461 atacaaaaca aaagcgctcc tcgtaccagc gaagaagggg cagagatgcc gtagtcaggt
     2521 ttagttcgtc cggcggcgcc agaaatccgc gcggtggttt ttgggggtcg ggggtgtttg
     2581 gcagccacag acgcccggtg ttcgtgtcgc gccagtacat gcggtccatg cccaggccat
     2641 ccaaaaacca tgggtctgtc tgctcagtcc agtcgtggac ctgaccccac gcaacgccca
     2701 aaagaataac ccccacgaac cataaaccat tccccatggg ggaccccgtc cctaacccac
     2761 ggggcccgtg gctatggcag ggcttgccgc cccgacgttg gctgcgagcc ctgggccttc
     2821 acccgaactt gggggttggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg
     2881 gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac
     2941 aaacgaccca acacccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc
     3001 cttccggtat tgtctccttc cgtgtttcag ttagcctccc ccatctcccg ggcaaacgtg
     3061 cgcgccaggt cgcagatcgt cggtatggag cctggggtgg tgacgtgggt ctggaccatc
     3121 ccggaggtaa gttgcagcag ggcgtcccgg cagccggcgg gcgattggtc gtaatccagg
     3181 ataaagacgt gcatgggacg gaggcgtttg gccaagacgt ccaaggccca ggcaaacacg
     3241 ttatacaggt cgccgttggg ggccagcaac tcgggggccc gaaacagggt aaataacgtg
     3301 tccccgatat ggggtcgtgg gcccgcgttg ctctggggct cggcaccctg gggcggcacg
     3361 gccgtccccg aaagctgtcc ccaatcctcc caccacgacc cgccgccctg cagataccgc
     3421 accgtattgg caagcagccc gtaaacgcgg cgaatcgcgg ccagcatagc caggtcaagc
     3481 cgctcgccgg ggcgctggcg tttggccagg cggtcgatgt gtctgtcctc cggaagggcc
     3541 cccaacacga tgtttgtgcc gggcaaggtc ggcgggatga gggccacgaa cgccagcacg
     3601 gcctgggggg tcatgctgcc cataaggtat cgcgcggccg ggtagcacag gagggcggcg
     3661 atgggatggc ggtcgaagat gagggtgagg gccgggggcg gggcatgtga actcccagcc
     3721 tcccccccga catgaggagc cagaacggcg tcggtcacgg cataaggcat gcccattgtt
     3781 atctgggcgc ttgtcattac caccgccgcg tccccggccg atatctcacc ctggtcgagg
     3841 cggtgttgtg tggtgtagat gttcgcgatt gtctcggaag cccccagcac ctgccagtaa
     3901 gtcatcggct cgggtacgta gacgatatcg tcgcgcgaac ccagggccac cagcagttgc
     3961 gtggtggtgg ttttccccat cccgtgagga ccgtctatat aaacccgcag tagcgtgggc
     4021 attttccgct ccaggcggac ttccgtggct tcttgctgcc ggcgagggcg caacgccgta
     4081 cgtcggttgc tatggccgcg agaacgcgca gcctggtcga acgcagacgc gtgttgatgg
     4141 caggggtacg aagccatggt gaattcgcgg atccgtcgaa actaagttct tggtgtttta
     4201 aaactaaaaa aaagactaac tataaaagta gaatttaaga agtttaagaa atagatttac
     4261 agaattacaa tcaataccta ccgtctttat atacttatta gtcaagtagg ggaataattt
     4321 cagggaactg gtttcaacct tttttttcag ctttttccaa atcagagaga gcagaaggta
     4381 atagaaggtg taagaaaatg agatagatac atgcgtgggt caattgcctt gtgtcatcat
     4441 ttactccagg caggttgcat cactccattg aggttgtgcc cgttttttgc ctgtttgtgc
     4501 ccctgttctc tgtagttgcg ctaagagaat ggacctatga actgatggtt ggtgaagaaa
     4561 acaatatttt ggtgctggga ttcttttttt ttctggatgc cagcttaaaa agcgggctcc
     4621 attatattta gtggatgcca ggaataaact gttcacccag acacctacga tgttatatat
     4681 tctgtgtaac ccgcccccta ttttgggcat gtacgggtta cagcagaatt aaaaggctaa
     4741 ttttttgact aaataaagtt aggaaaatca ctactattaa ttatttacgt attctttgaa
     4801 atggcagtat tgataatgat aaactcgacc tgcagccaag cttatcgatg ataagctgtc
     4861 aaacatgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc
     4921 actagttcta gctagaggat cctaaaacaa gaagagggtt gactacatca cgatgagggg
     4981 gatcgaagaa atgatggtaa atgaaatagg aaatcaagga gcatgaaggc aaaagacaaa
     5041 tataagggtc gaacgaaaaa taaagtgaaa agtgttgata tgatgtattt ggctttgcgg
     5101 cgccgaaaaa acgagtttac gcaattgcac aatcatgctg actctgtggc ggacccgcgc
     5161 tcttgccggc ccggcgataa cgctgggcgt gaggctgtgc ccggcggagt tttttgcgcc
     5221 tgcattttcc aaggtttacc ctgcgctaag gggcgagatt ggagaagcaa taagaatgcc
     5281 ggttggggtt gcgatgatga cgaccacgac aactggtgtc attatttaag ttgccgaaag
     5341 aacctgagtg catttgcaac atgagtatac tagaagaatg agccaagact tgcgagacgc
     5401 gagtttgccg gtggtgcgaa caatagagcg accatgacct tgaaggtgag acgcgcataa
     5461 ccgctagagt actttgaaga ggaaacagca atagggttgc taccagtata aatagacagg
     5521 tacatacaac actggaaatg gttgtctgtt tgagtacgct ttcaattcat ttgggtgtgc
     5581 actttattat gttacaatat ggaagggaac tttacacttc tctaagcaca tatattaatt
     5641 aaagtccaat gctagtagag aaggggggta acacccctcc gcgctctttt ccgatttttt
     5701 tctaaaccgt ggaatatttc ggatatcctt ttgttgtttc cgggtgtaca atatggactt
     5761 cctcttttct ggcaaccaaa acccaacatc ggggattcct ataatacctt cggtggtctc
     5821 cctaacatgt aggtggcgga ggggagatat catagaacag atcccagcaa gacataatgg
     5881 gctaaacaaa gactacacca attacactgc ctcattgatg gtggtacata acgaactaat
     5941 actgtagccc tagacttgat agccatcatc atatcgaagt ttcactaccc tttttccatt
     6001 tgccatctat tgaagtaata ataggcgcat gcaacttctt ttcttttttt ttcttttctc
     6061 tctcccccgt tgttgtctca ccatatccgc aatgacaaaa aaatgatgga agacactaaa
     6121 ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg
     6181 gtatctcgaa gcacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga
     6241 gcaacggtat acggccttcc ttccagttac ttgaatttga aataaaaaaa agtttgctgt
     6301 cttgctatca agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc
     6361 tcgttccctt tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac
     6421 aatcaactcc aagctcggaa ttcgatatca agcttatcga taagcttgat atcgaattcc
     6481 tgcagcccgg gggatctatg acaaccagtc accagcctca ggacagatac aaagctgtct
     6541 ggcttatctt cttcatgctg ggtctgggaa cgctgctccc gtggaatttt ttcatgacgg
     6601 ccactcagta tttcacaaac cgcctggaca tgtcccagaa tgtgtccttg gtcactgctg
     6661 aactgagcaa ggacgcccag gcgtcagccg cccctgcagc acccttgcct gagcggaact
     6721 ctctcagtgc catcttcaac aatgtcatga ccctatgtgc catgctgccc ctgctgttat
     6781 tcacctacct caactccttc ctgcatcaga ggatccccca gtccgtacgg atcctgggca
     6841 gcctggtggc catcctgctg gtgtttctga tcactgccat cctggtgaag gtgcagctgg
     6901 atgctctgcc cttctttgtc atcaccatga tcaagatcgt gctcattaat tcatttggtg
     6961 ccatcctgca gggcagcctg tttggtctgg ctggccttct gcctgccagc tacacggccc
     7021 ccatcatgag tggccagggc ctagcaggct tctttgcctc cgtggccatg atctgcgcta
     7081 ttgccagtgg ctcggagcta tcagaaagtg ccttcggcta ctttatcaca gcctgtgctg
     7141 ttatcatttt gaccatcatc tgttacctgg gcctgccccg cctggaattc taccgctact
     7201 accagcagct caagcttgaa ggacccgggg agcaggagac caagttggac ctcattagca
     7261 aaggagagga gccaagagca ggcaaagagg aatctggagt ttcagtctcc aactctcagc
     7321 ccaccaatga aagccactct atcaaagcca tcctgaaaaa tatctcagtc ctggctttct
     7381 ctgtctgctt catcttcact atcaccattg ggatgtttcc agccgtgact gttgaggtca
     7441 agtccagcat cgcaggcagc agcacctggg aacgttactt cattcctgtg tcctgtttct
     7501 tgactttcaa tatctttgac tggttgggcc ggagcctcac agctgtattc atgtggcctg
     7561 ggaaggacag ccgctggctg ccaagcctgg tgctggcccg gctggtgttt gtgccactgc
     7621 tgctgctgtg caacattaag ccccgccgct acctgactgt ggtcttcgag cacgatgcct
     7681 ggttcatctt cttcatggct gcctttgcct tctccaacgg ctacctcgcc agcctctgca
     7741 tgtgcttcgg gcccaagaaa gtgaagccag ctgaggcaga gaccgcagga gccatcatgg
     7801 ccttcttcct gtgtctgggt ctggcactgg gggctgtttt ctccttcctg ttccgggcaa
     7861 ttgtgtgact cgaggcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga
     7921 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7981 ccgctcacaa ttccacacaa cataggagcc ggaagcataa agtgtaaagc ctggggtgcc
     8041 taatgagtga ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8101 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8161 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8221 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8281 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8341 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8401 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8461 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8521 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8581 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8641 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8701 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8761 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8821 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8881 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8941 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9001 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9061 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9121 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9181 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9241 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9301 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9361 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9421 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9481 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9541 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9601 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9661 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9721 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9781 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9841 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9901 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9961 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10021 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10081 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    10141 aaaaataggc gtatcacgag gccctttcgt c
//
