LOCUS       sequence-137663-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of secreted mMBP TEV cleavage site-linked 
            fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Bokhove M, Sadat Al Hosseini H, Saito T, Dioguardi E, 
            Gegenschatz-Schmid K, Nishimura K, Raj I, de Sanctis D, Han L, 
            Jovine L
  TITLE     Easy mammalian expression and crystallography of maltose-binding 
            protein-fused human proteins.
  JOURNAL   J Struct Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi:
            10.1016/j.jsb.2016.01.016.
  PUBMED    26850170
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi: 
            10.1016/j.jsb.2016.01.016."
COMMENT     Sequence Label: pHLmMBP-11
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagaattgcg gccgtctcag gccgaattcg tcgacaagct tgccaccatg gggatccttc
       61 ccagccctgg gatgcctgcg ctgctctccc tcgtgagcct tctctccgtg ctgctgatgg
      121 gttgcgtagc tgaaaccggt accaaaatcg aagaaggtaa actggtaatc tggattaacg
      181 gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa
      241 ttaaagtcac cgttgagcat cccgataaac tggaagagaa attcccacag gttgcggcaa
      301 ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat
      361 ctggcctgtt ggctgaaatc accccggccg cagcgttcca ggacaagctg tatccgttta
      421 cctgggatgc cgtgcgctac aacggcaagc tgattgctta cccgatcgct gttgaagcgt
      481 tgtccctgat ttataacaag gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc
      541 cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag
      601 aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgcag
      661 ccggaaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga
      721 ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg
      781 cagaacatgc ctttaatcat ggcgaaacag cgatgaccat caacggcccg tgggcatggt
      841 ccaacatcga caccagcgca gtgaattatg gtgtaacggt gctgccgacc ttcaagggtc
      901 aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca
      961 aagagctggc aaaagagttc ctcgaaa
//
