LOCUS       sequence-137682-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible sucrose counter-selection allelic exchange vector
            for Pseudomonas syringae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Mijatovic J, Severns PM, Kemerait RC, Walcott RR, Kvitko BH
  TITLE     Patterns of Seed-to-Seedling Transmission of Xanthomonas citri pv. 
            malvacearum, the Causal Agent of Cotton Bacterial Blight.
  JOURNAL   Phytopathology. 2021 Dec;111(12):2176-2184. doi: 
            10.1094/PHYTO-02-21-0057-R. Epub 2021 Nov 22.
  PUBMED    34032522
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/PHYTO-02-21-0057-R"; journalName: "Phytopathology"; date: 
            "2021-12"; volume: "111"; issue: "12"; pages: "2176-2184"
COMMENT     Sequence Label: pDONR1K18ms
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..273
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        418..520
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(587..606)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cctcgatttt gagacacggg ccagagctgc agctggatgg caaataatga ttttattttg
       61 actgatagtg acctgttcgt tgcaacaaat tgataagcaa tgctttctta taatgccaac
      121 tttgtacaag aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
      181 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
      241 aatcaactac ttagatggta ttagtgacct gtagtcgact aagttggcag catcacccga
      301 cgcactttgc gccgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc
      361 tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga
      421 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      481 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      541 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      601 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      661 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      721 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
      781 tgttcacc
//
