LOCUS       sequence-137696-        7117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Abies grandis PHLS as a fusion with the CpcB protein 
            plus the CmR cassete in Synechocystis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Formighieri C, Melis A
  TITLE     Cyanobacterial production of plant essential oils.
  JOURNAL   Planta. 2018 Oct;248(4):933-946. doi: 10.1007/s00425-018-2948-0. 
            Epub 2018 Jul 4.
  PUBMED    29974209
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00425-018-2948-0"; journalName: "Planta"; date: "2018-10";
            volume: "248"; issue: "4"; pages: "933-946"
COMMENT     Sequence Label: CpcB-Abies-grandisPHLS_CpcA
FEATURES             Location/Qualifiers
     source          1..7117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3508..3610
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3611..4270
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3677..3696)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4932..4950)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4971..4987)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4971..4987)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4984..5006)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4995..5011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5019..5025,5026..5043,5044..5049))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5064..5085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5202..5219)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5373..5961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5453..5472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6132..6923,6924..6992))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6755..6774
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6993..7097)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gagaagagtc cctgaatatc aaaatggtgg gataaaaagc tcaaaaagga
      721 aagtaggctg tggttcccta ggcaacagtc ttccctaccc cactggaaac taaaaaaacg
      781 agaaaagttc gcaccgaaca tcaattgcat aattttagcc ctaaaacata agctgaacga
      841 aactggttgt cttcccttcc caatccagga caatctgaga atcccctgca acattactta
      901 acaaaaaagc aggaataaaa ttaacaagat gtaacagaca taagtcccat caccgttgta
      961 taaagttaac tgtgggattg caaaagcatt caagcctagg cgctgagctg tttgagcatc
     1021 ccggtggccc ttgtcgctgc ctccgtgttt ctccctggat ttatttaggt aatatctctc
     1081 ataaatcccc gggtagttaa cgaaagttaa tggagatcag taacaataac tctagggtca
     1141 ttactttgga ctccctcagt ttatccgggg gaattgtgtt taagaaaatc ccaactcata
     1201 aagtcaagta ggagattaat tcaatgttcg acgtattcac tcgggttgtt tcccaagctg
     1261 atgctcgcgg cgagtacctc tctggttctc agttagatgc tttgagcgct accgttgctg
     1321 aaggcaacaa acggattgat tctgttaacc gcatcaccgg taatgcttcc gctatcgttt
     1381 ccaacgctgc tcgtgctttg ttcgccgaac agccccaatt aatccaaccc ggtggaaacg
     1441 cctacaccag ccgtcgtatg gctgcttgtt tgcgtgacat ggaaatcatc ctccgctatg
     1501 ttacctacgc aaccttcacc ggcgacgctt ccgttctaga agatcgttgc ttgaacggtc
     1561 tccgtgaaac ctacgttgcc ctgggtgttc ccggtgcttc cgtagctgct ggcgttcaaa
     1621 aaatgaaaga agctgccctg gacatcgtta acgatcccaa tggcatcacc cgtggtgatt
     1681 gcagtgctat cgttgctgaa atcgctggtt acttcgaccg cgccgctgct gccgtagccc
     1741 atatgtcctt gaccacagcc gtgagcgatg atgggctgca gagaagaatt ggcgactatc
     1801 attccaattt gtgggatgac gattttatac aatccctttc gacaccgtat ggagaaccta
     1861 gttaccgtga acgcgccgaa aagctgattg gagaagtaaa agaaatgttt aattccatgc
     1921 cctccgagga tggtgaaagt atgagtcctc tcaacgactt gattgaacgt ttgtggatgg
     1981 tggattccgt cgagcgcttg ggaattgacc gtcattttaa aaaagaaatt aagagcgctt
     2041 tggactatgt gtacagctat tggaatgaga aaggcattgg ttgcggccga gatagcgttt
     2101 ttccagatgt gaatagtact gctagcggat ttcggacttt gcggctacat ggctattccg
     2161 tatctagcga ggtgttaaag gtattccaag atcaaaatgg tcagttcgca ttttccccta
     2221 gtaccaaaga gcgcgacata cgcactgtcc taaacctcta ccgggcctca ttcatcgcct
     2281 ttccaggtga gaaagtaatg gaagaggctg aaatttttag tagtcgctat ttgaaagaag
     2341 ctgtgcaaaa gatccccgtt agtagcctca gtcaagaaat tgactatact ttggaatatg
     2401 ggtggcatac caatatgcct cggttggaaa ctcgcaatta cctagacgtg tttggccacc
     2461 ccacttcacc gtggttaaaa aagaaacgca cacaatatct agacagtgag aaactcctgg
     2521 aattagctaa gctagaattc aatatctttc attcattaca acaaaaggag ttacaatatc
     2581 tgtcccgatg gtggattcac tcaggcctgc cggaattgac gtttggtcga catcggcatg
     2641 tcgaatacta caccctctct agctgtattg ccaccgagcc caagcatagt gcttttcgcc
     2701 tcgggttcgc caagacttgt cacctaatta ccgttttgga tgatatctac gatacgtttg
     2761 gaacgatgga tgagatcgaa ttgtttaatg aagcagttcg gcggtggaat ccttctgaga
     2821 aagaacggct ccccgaatat atgaaggaga tatatatggc actttacgaa gctctgaccg
     2881 acatggcacg tgaggccgaa aaaactcagg gccgtgacac tctgaactac gcgcgtaaag
     2941 cttgggaagt ttacctcgat tcatataccc aggaggcgaa gtggatcgct tccgggtatt
     3001 tgcccacctt cgaagagtac cttgaaaatg ccaaagttag ttccggtcac cgggctgcgg
     3061 cacttacccc gttattaacc ctcgacgtcc ccttgccgga tgatgtgctg aaaggcattg
     3121 actttccctc ccgttttaac gacttagcgt ctagcttctt gcggctccgg ggtgacaccc
     3181 gctgttataa agccgatcgc gatcgaggag aggaagccag ttcaatctct tgttatatga
     3241 aggataatcc gggcctaacc gaagaagatg cgctcaatca tattaatgcg atgattaacg
     3301 acataattaa ggaattaaac tgggaattgt tgaaaccaga ttccaacatt ccgatgacag
     3361 ctcgcaaaca cgcgtatgaa attacgcgcg cttttcatca actctacaaa tatcgcgatg
     3421 gattcagcgt tgccactcaa gaaaccaaaa gccttgttcg gcgtaccgta ttagaaccag
     3481 tgcccttata gagatctgcg gccgcgttga tcggcacgta agaggttcca actttcacca
     3541 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     3601 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     3661 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     3721 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     3781 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     3841 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     3901 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     3961 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     4021 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     4081 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     4141 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga
     4201 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     4261 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctgggg atcctctggt
     4321 tattttaaaa accaacttta ctcaggttcc atacccgaga aaatccagct taaagctgac
     4381 atatctagga aaattttcac attctaacgg gagataccag aacaatgaaa acccctttaa
     4441 ctgaagccgt ttccaccgct gactctcaag gtcgctttct gagcagcacc gaattgcaaa
     4501 ttgctttcgg tcgtctacgt caagctaatg ctggtttgca agccgctaaa gctctgaccg
     4561 acaatgccca gagcttggta aatggtgctg cccaagccgt ttataacaaa ttcccctaca
     4621 ccacccaaac ccaaggcaac aactttgctg cggatcaacg gggtaaagac aagtgtgccc
     4681 gggacatcgg ctactacctc cgcatcgtta cctactgctt agttgctggt ggtaccggtc
     4741 ctttggatga gtacttgatc gccggtattg atgaaatcaa ccgcaccttt gacctctccc
     4801 ccagctggta tgttgaagct ctgaaataca tcaaagctaa ccacggcttg agtggcgatg
     4861 cccgtgacga agctaattcc tacctcgatt acgccatcaa tgctctgagc taggagctcc
     4921 agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg
     4981 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5041 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5101 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     5161 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5221 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5281 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     5821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6421 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6721 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6961 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7021 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7081 caaatagggg ttccgcgcac atttccccga aaagtgc
//
