LOCUS       sequence-137742-         635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Acetobacter aceti 1023 gDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 ORFs associated with functional 
            complementation of an alanine racemase (alr-, dadX-) auxotroph
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK215
FEATURES             Location/Qualifiers
     source          1..635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(30..63)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        70..88
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gccgattcat taatgcagcc ccggtaccca taacttcgta tagcatacat tatacgaagt
       61 tatgcggcca attaaccctc actaaaggga gcggccgcgg atcattaaac cgaatgttca
      121 gccgggcttc ggcccgtgcg ggaataacat tggtcgcccc attgcctaca tccacactgg
      181 taacctgaag gctggagggt tcaaaatact cggtaccgtt atccagcggt gtggcgcgca
      241 acgcttccag caccgccagc aggcgatgca ccgggttatc tgcccggtgg ggataggcca
      301 catggccctg cgtaccttcc accacaatat gcgcgttcaa gctgccacgg cggccaacct
      361 tcaccatctc gcccaacacc ttggggttgg tgggttcacc taccagacag taatccggga
      421 tctgattgtg ctgcgccatc cattccagca cctttaccgt gccataggtg gcggggcctt
      481 cttcatcccc ggtgatcaga aaactgatgg agcctttgcc cggcccagcc gccacatgcc
      541 gcgctacggc agatacaaaa gccgcaatac cgcccttcat gtcacacgcg ccacggccat
      601 acagcacgcc atctacaata tccgccgcat aaggg
//
