LOCUS       sequence-137778-         468 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible sucrose counter-selection allelic exchange vector
            for Pseudomonas syringae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 468)
  TITLE     Kvitko lab Gateway compatible allelic exchange plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDEST2T18ms
FEATURES             Location/Qualifiers
     source          1..468
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(106..411)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(208..227)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
       61 ctatggtcga cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa
      121 catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc
      181 cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc
      241 cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact
      301 ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac
      361 aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc
      421 ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcga
//
