LOCUS       sequence-137780-        2808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry clone for synthetic TetO2-CYC1TATA promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Giuraniuc CV, MacPherson M, Saka Y
  TITLE     Gateway vectors for efficient artificial gene assembly in vitro and 
            expression in yeast Saccharomyces cerevisiae.
  JOURNAL   PLoS One. 2013 May 10;8(5):e64419. doi: 
            10.1371/journal.pone.0064419. Print 2013.
  PUBMED    23675537
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-10"; volume: "8(5):e64419. doi"; pages: " 
            10.1371/journal.pone.0064419. Print 2013"
COMMENT     Sequence Label: pYS7
FEATURES             Location/Qualifiers
     source          1..2808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    570..665
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    840..858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    882..900
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(920..1019)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1049..1068)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1050..1068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1073..1089)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1073..1089)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1191..1210)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1202..2011
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1269..1288)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2158..2746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2647..2666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa
      661 agttgtaatt tagtgtgtgt atttgtgttt gcgtgtctat agaagtatag taatttatgc
      721 tacaaaggac ctaatgtata aggaaagaat atttagagaa aagaagaaaa caagagtttt
      781 atatacatac agagcacatg catgcagtgg tctcgatcga gaaactcgac tttgtcgact
      841 ctctatcact gatagggatc gagaaactcg actttgtcga ctctctatca ctgataggga
      901 tcgaggaatt gatctgccta cccagctttc ttgtacaaag ttggcattat aagaaagcat
      961 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     1021 catccagctg cagggcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc
     1081 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     1141 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     1201 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     1261 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1321 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1381 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     1441 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1501 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1561 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     1621 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     1681 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     1741 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     1801 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     1861 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     1921 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     1981 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     2041 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     2101 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2161 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2221 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2281 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2341 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2401 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2461 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2521 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
