LOCUS       sequence-137802-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
  TITLE     Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron 
            Tagging with Short Homology Donors.
  JOURNAL   Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
            Epub 2016 Mar 24.
  PUBMED    27052166
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.001"; journalName: "Cell Rep"; date: 
            "2016-04-5- 5"; volume: "15"; issue: "1"; pages: "210-8"
COMMENT     Sequence Label: pMK285 (Stag-3FLAG-Bsr)
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctgttctcct cttcctcatc tccgggcctt tcgacctgca tccatctaga tctcgagcag
       61 ctgaagctta ccgctagcat gaaaacattt aacatttctc aacaagatct agaattagta
      121 gaagtagcga cagagaagat tacaatgctt tatgaggata ataaacatca tgtgggagcg
      181 gcaattcgta cgaaaacagg agaaatcatt tcggcagtac atattgaagc gtatatagga
      241 cgagtaactg tttgtgcaga agccattgcg attggtagtg cagtttcgaa tggacaaaag
      301 gattttgaca cgattgtagc tgttagacac ccttattctg acgaagtaga tagaagtatt
      361 cgagtggtaa gtccttgtgg tatgtgtagg gagttgattt cagactatgc accagattgt
      421 tttgtgttaa tagaaatgaa tggcaagtta gtcaaaacta cgattgaaga actcattcca
      481 ctcaaatata cccgaaatta agctagccgc ccgccccacg acccgcagcg cccgaccgaa
      541 aggagcgcac gaccccatgc atcgatgata tcagatcccc gggatgcaga aattgatgat
      601 ctattaaaca ataaagatgt ccactaaaat ggaagttttt cctgtcatac tttgttaaga
      661 agggtgagaa cagagtacct acattttgaa tggaaggatt ggagctacgg gggtgggggt
      721 ggggtgggat t
//
