LOCUS       sequence-137942-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial pMCSG7 expression vector expressing catalytically inactive
            Nme cas9 with T7 promoter, N-terminal His tag and TEV site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Zhang Y, Rajan R, Seifert HS, Mondragon A, Sontheimer EJ
  TITLE     DNase H Activity of Neisseria meningitidis Cas9.
  JOURNAL   Mol Cell. 2015 Oct 15;60(2):242-55. doi: 
            10.1016/j.molcel.2015.09.020.
  PUBMED    26474066
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.09"; journalName: "Mol Cell"; date: 
            "2015-10-15- 15"; volume: "60"; issue: "2"; pages: "242-55"
COMMENT     Sequence Label: pMCSG7-dNmeCas9
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcgtgatggc ggtcgttttc cgcacgcact ttgcgcaatc cccaaaagcc gcgcaacaga
       61 ttggtaattt gtccgttgga tgcaaagaca cgtttcttgc ctttacctgt cagccgcata
      121 cggtcggcaa caaattgaca caggaaacgg ttgacgtagc gcgtgtcgtt cagattgcgt
      181 tctttaaagc cgtcttcatc gaatttttgc agcagaatcc gttgtttttt actgcgcggg
      241 aaacggctgg tttcgacacg cgctttaaat tcctgccatt cgcggctgtt gtctttgccg
      301 ttgaagtatt cgtaaggggt ttgattgcct ttgttttggt tttcgctgcc caataccagt
      361 actttattgt tgaaactgtc gtcccatgtg cgcgagaacg gcagggcagc gtcgatttcg
      421 acatagcctt tttcgttcag acggcctaag ttgatttctt tgcccgaata caggcatttg
      481 ccgtgttgtt gctcgtacag gcgcagtttc agaatatctt tggatttggg ttctccgaca
      541 aaattgggga aatactctcg gaatttggcg gcggcttttt cccggtcttt gcggttttct
      601 tcttggcgtt tctcaatttc tttgcggtct ttaaacgatt tacctacttc ccttgcagtt
      661 tcaatatgga tacgagctgg ggagccgtaa cggcgtacca cgccgttaat gaccttacgt
      721 gcttga
//
