LOCUS       sequence-137951-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construction of ycrtE gene in HCKan-O receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-O-ycrtE
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(28..130)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(131..162)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 agacgtttcc cgttgaatat ggctcatttt agcttcctta gctcctgaaa atctcgataa
       61 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      121 gtgccgatca agtcaaaagc ctccggtcgg aggcttttga ctttctgcta tggaggtcag
      181 gtatgattta aatggtcagt cgtctcggct acaatgggat gtcagccaac ttcttcaaga
      241 tagcttccaa cttaacgttc ttaccggttc taccttcaga gtcaacgtca cccaagtcca
      301 ttctactgtt agcttcagcg aactcacctt gcaatctacc caattctctt tccaaagcac
      361 cagacaaggt gttcaaaact tcttgggtgt attcgaaaga gtgggtttcg gttctcatgt
      421 agttaacaca gtggtgcaag atttctggag aggtagactt cttttgcaag gtgttgataa
      481 ccaatctact agatgggtta gcgtggatag agtggatggt tgggaaagag aacttacctt
      541 cggtcaagtc ttcagcgaag ttcttgttgt gagcgtattc agaagattgc aagttcatgt
      601 agtcgtcgcg gatttggaag tagatagaga tcaagttaac caattgaacg aagtcgatgt
      661 cacattcaga cttagccatc atcaatctaa cagcgattct gaacaaacca ccggtcttac
      721 c
//
