LOCUS       sequence-137964-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Vo BT, Wolf E, Kawauchi D, Gebhardt A, Rehg JE, Finkelstein D, Walz 
            S, Murphy BL, Youn YH, Han YG, Eilers M, Roussel MF
  TITLE     The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup 
            Identity.
  JOURNAL   Cancer Cell. 2016 Jan 11;29(1):5-16. doi: 
            10.1016/j.ccell.2015.12.003.
  PUBMED    26766587
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2015.12"; journalName: "Cancer Cell"; date: 
            "2016-01-11- 11"; volume: "29"; issue: "1"; pages: "5-16"
COMMENT     Sequence Label: pCL20-miR30-shRNA#2 IFT88-PGK-mcherry
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           39..161
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           189..203
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           231..357
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(418..441)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ctccttctct aggcgccgga attagatctc tcgactgttt gaatgaggct tcagttactt
       61 tacagaatcg ttgcctgcac atcttggaaa cacttgctgg gattacttct tcaggttaac
      121 ccaacagaag gctcgagaag gtatattgct gttgacagtg agcgctagaa attgatgaag
      181 atgatatagt gaagccacag atgtatatca tcttcatcaa tttctaatgc ctactgcctc
      241 ggaattcaag gggctacttt aggagcaatt atcttgttta ctaaaactga ataccttgct
      301 atctctttga tacattttta caaagctgaa ttaaaatggt ataaattaaa tcactttttt
      361 cctaggacgc gtcaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
      421 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
      481 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
      541 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
      601 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
      661 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
      721 tgggctcaga ggctgggaag gg
//
