LOCUS       sequence-138000-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express Assembly-activating protein (AAP) of adeno-associated virus 
            serotype 2 (AAV2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Zinn E, Pacouret S, Khaychuk V, Turunen HT, Carvalho LS, 
            Andres-Mateos E, Shah S, Shelke R, Maurer AC, Plovie E, Xiao R, 
            Vandenberghe LH
  TITLE     In Silico Reconstruction of the Viral Evolutionary Lineage Yields a 
            Potent Gene Therapy Vector.
  JOURNAL   Cell Rep. 2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 
            10.1016/j.celrep.2015.07.019.
  PUBMED    26235624
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 
            10.1016/j.celrep.2015.07.019."
COMMENT     Sequence Label: pAAP2
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             140..934
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(194..213)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     804..823
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
       61 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
      121 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
      181 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
      241 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
      301 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
      361 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
      421 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
      481 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
      541 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
      601 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
      661 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
      721 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
      781 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
      841 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
      901 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
      961 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     1021 aggttgggct tcg
//
