LOCUS       sequence-138002-         463 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains dCas9 from S. pyogenes under aTc inducible promoter. 
            Suicide vector inserts into psba1 site of Synechocystis. Carries 
            spectinomycin resist. Recommend E. coli Copy cutter for 
            propogation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-psba1-TetR-PL22-dCas9-SpR
FEATURES             Location/Qualifiers
     source          1..463
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(55..82)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(98..169)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(189..218)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agtcggcaaa taatgtctaa caattcgttc aagccgaggg ctctagtata taaacgcaga
       61 aaggcccacc cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac
      121 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggctc
      181 tagtattaca aatcttcttc actaatcaat ttttgttcgt cacctcctag ctgactcaaa
      241 tcaatgcgtg tttcataaag accagtgatg gattgatgga taagagtggc atctaaaact
      301 tcttttgtag acgtatatcg tttacgatca attgttgtat caaaatattt aaaagcagcg
      361 ggagctccaa gattcgtcaa cgtaaataaa tgaataatat tttctgcttg ttcacgtatt
      421 ggtttgtctc tatgtttgtt atatgcacta agaactttat cta
//
