LOCUS       sequence-138011-         559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains dCas9 from S. pyogenes under aTc inducible promoter. 
            Suicide vector inserts into psba1 site of Synechocystis. Carries 
            spectinomycin resist. Recommend E. coli Copy cutter for 
            propogation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-psba1-TetR-PL22-dCas9-SpR
FEATURES             Location/Qualifiers
     source          1..559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    190..208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    215..233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(248..275)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(291..362)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(410..442)
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
ORIGIN
        1 taaagagccc ctataagatt ttttttgata ctgtggcggt ctgtatttcc cagaaccttg
       61 aactttttag acggaacctt atattcatca gtgatcaccg cccatccgac gctatttgtg
      121 ccgatagcta agcctattga gtatttctta tccatctagt aacctccact actctagtag
      181 ctcccagtat ctctatcact gatagggatg tcaatctcta tcactgatag ggactctagt
      241 atataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc
      301 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
      361 tgatgcctgg ctctagtagt gatctacact agcactatca gtgttattaa gctactaaag
      421 cgtagttttc gtcgtttgca gcggacccac tttcacattt aagttgtttt tctaatccgc
      481 atatgatcaa ttcaaggccg aataagaagg ctggctctgc accttggtga tcaaataatt
      541 cgatagcttg tcgtaataa
//
