LOCUS       sequence-138030-         370 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the LexA Binding Domain fused to SRDX Transcriptional 
            Repressor as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pLexA::SRDX (GB0732)
FEATURES             Location/Qualifiers
     source          1..370
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..290
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
ORIGIN
        1 cggccgcggg aattcgattg ggcgatgagt ggtctcgaat gaaagcgtta acggccaggc
       61 aacaagaggt gtttgatctc atccgtgatc acatcagcca gacaggtatg ccgccgacgc
      121 gtgcggaaat cgcgcagcgt ttggggttcc gttccccaaa cgcggctgaa gaacatctga
      181 aggcgctggc acgcaaaggc gttattgaaa ttgtttccgg cgcatcacgc gggattcgtc
      241 tgttgcagga agaggaagaa gggttgccgc tggtaggtcg tgtggctgcc ggtgaaccgg
      301 agccacttga tcttgatctt gaacttagac ttggttttgc ttaggcttcg agaccactca
      361 tcgcccaatc
//
