LOCUS       sequence-138032-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the LexA Binding Domain fused to SRDX Transcriptional 
            Repressor as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pLexA::SRDX (GB0732)
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..383)
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     regulatory      418..427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      422..431
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(461..480)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(462..480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(487..504)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(487..503)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(496..518)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(731..750)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttgggagctc tcccatatgg tcgacctgca ggcggccgcg aattcactag tgattgggcg
       61 atgagtggtc tcgaagccta agcaaaacca agtctaagtt caagatcaag atcaagtggc
      121 tccggttcac cggcagccac acgacctacc agcggcaacc cttcttcctc ttcctgcaac
      181 agacgaatcc cgcgtgatgc gccggaaaca atttcaataa cgcctttgcg tgccagcgcc
      241 ttcagatgtt cttcagccgc gtttggggaa cggaacccca aacgctgcgc gatttccgca
      301 cgcgtcggcg gcatacctgt ctggctgatg tgatcacgga tgagatcaaa cacctcttgt
      361 tgcctggccg ttaacgcttt cattcgagac cactcatcgc ccaatcgaat tcccgcggcc
      421 gccatggcgg ccgggagcat gcgacgtcgg gcccaattcg ccctatagtg agtcgtatta
      481 caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      541 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      601 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
      661 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      721 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      781 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      841 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggcca
//
