LOCUS       sequence-138102-        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For recombinant expression of human GEC1/GABARAPL1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Ma P, Schwarten M, Schneider L, Boeske A, Henke N, Lisak D, Weber S,
            Mohrluder J, Stoldt M, Strodel B, Methner A, Hoffmann S, Weiergraber
            OH, Willbold D
  TITLE     Interaction of Bcl-2 with the autophagy-related GABAA 
            receptor-associated protein (GABARAP): biophysical characterization 
            and functional implications.
  JOURNAL   J Biol Chem. 2013 Dec 27;288(52):37204-15. doi: 
            10.1074/jbc.M113.528067. Epub 2013 Nov 15.
  PUBMED    24240096
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.528067"; journalName: "J Biol Chem"; date: 
            "2013-12-27- 27"; volume: "288"; issue: "52"; pages: "37204-15"
COMMENT     Sequence Label: pGex-4T-2_GEC1
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..669
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     627..649
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             676..693
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 caggaaacag tattcatgtc ccctatacta ggttattgga aaattaaggg ccttgtgcaa
       61 cccactcgac ttcttttgga atatcttgaa gaaaaatatg aagagcattt gtatgagcgc
      121 gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg gtttggagtt tcccaatctt
      181 ccttattata ttgatggtga tgttaaatta acacagtcta tggccatcat acgttatata
      241 gctgacaagc acaacatgtt gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt
      301 gaaggagcgg ttttggatat tagatacggt gtttcgagaa ttgcatatag taaagacttt
      361 gaaactctca aagttgattt tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat
      421 cgtttatgtc ataaaacata tttaaatggt gatcatgtaa cccatcctga cttcatgttg
      481 tatgacgctc ttgatgttgt tttatacatg gacccaatgt gcctggatgc gttcccaaaa
      541 ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa ttgataagta cttgaaatcc
      601 agcaagtata tagcatggcc tttgcagggc tggcaagcca cgtttggtgg tggcgaccat
      661 cctccaaaat cggatctggt tccgcgtgga tccatgaagt tccagtacaa ggaggaccat
      721 ccctttgagt atcggaaaaa ggaaggagaa aagatccgga agaaatatcc ggacagggtc
      781 cccgtgattg tagagaaggc tccaaaagcc agggtgcctg atctggacaa gaggaagtac
      841 ctagtgccct ctgaccttac tgttggccag ttctacttct taatccggaa gagaatccac
      901 ctgagacctg aggacgcctt attcttcttt gtcaacaaca ccatccctcc caccagtgct
      961 accatgggcc aactgtatga ggacaatcat gaggaagact attttctgta tgtggcctac
     1021 agtgatgaga gtgtctatgg gaaataagcg gccgcatcgt gactgactga cgatctgcct
     1081 cgcgcgtttc ggtgatgac
//
