LOCUS       sequence-138103-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For recombinant expression of human GATE-16/GABARAPL2 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma P, Schwarten M, Schneider L, Boeske A, Henke N, Lisak D, Weber S,
            Mohrluder J, Stoldt M, Strodel B, Methner A, Hoffmann S, Weiergraber
            OH, Willbold D
  TITLE     Interaction of Bcl-2 with the autophagy-related GABAA 
            receptor-associated protein (GABARAP): biophysical characterization 
            and functional implications.
  JOURNAL   J Biol Chem. 2013 Dec 27;288(52):37204-15. doi: 
            10.1074/jbc.M113.528067. Epub 2013 Nov 15.
  PUBMED    24240096
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.528067"; journalName: "J Biol Chem"; date: 
            "2013-12-27- 27"; volume: "288"; issue: "52"; pages: "37204-15"
COMMENT     Sequence Label: pGex-4T-2_GATE-16
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     602..624
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             651..668
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tactaggtta ttggaaaatt aagggccttg tgcaacccac tcgacttctt ttggaatatc
       61 ttgaagaaaa atatgaagag catttgtatg agcgcgatga aggtgataaa tggcgaaaca
      121 aaaagtttga attgggtttg gagtttccca atcttcctta ttatattgat ggtgatgtta
      181 aattaacaca gtctatggcc atcatacgtt atatagctga caagcacaac atgttgggtg
      241 gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg agcggttttg gatattagat
      301 acggtgtttc gagaattgca tatagtaaag actttgaaac tctcaaagtt gattttctta
      361 gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt atgtcataaa acatatttaa
      421 atggtgatca tgtaacccat cctgacttca tgttgtatga cgctcttgat gttgttttat
      481 acatggaccc aatgtgcctg gatgcgttcc caaaattagt ttgttttaaa aaacgtattg
      541 aagctatccc acaaattgat aagtacttga aatccagcaa gtatatagca tggcctttgc
      601 agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat ctggttccgc
      661 gtggatccat gaagtggatg ttcaaggagg accactcgct ggaacacaga tgcgtggagt
      721 ccgcgaagat tcgagcgaaa tatcccgaca gggttccggt gattgtggaa aaggtctcag
      781 gctctcagat tgttgacatt gacaaacgga agtacttggt tccatctgat atcactgtgg
      841 ctcagttcat gtggatcatc aggaaaagaa tccagcttcc ttctgaaaag gcgatcttcc
      901 tgtttgtgga taagacagtc ccacagtcca gcctaactat gggacagctt tacgagaagg
      961 aaaaagatga agatggattc ttatatgtgg cctacagcgg agagaacact tttggcttct
     1021 aagcggccgc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga t
//
