LOCUS       sequence-138129-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains sgRNA which targets GFPmut3b. Under an aTc inducible 
            promoter. Suicide vector inserts into slr0230 site of Synechocystis.
            Carries kanamycin resistance. Propogates in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-slr0230-PL31-sgRNANT1-KmR
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgccacctga cgtctaagaa accataaccg ttgtcccttt tgtttcatcg accaacaacc
       61 gccggggaaa cgggagagtt tgtattacaa agatgatgac tatcgcctga gttttctcta
      121 cggcagttat ctcactttga ctaatttgag tgccaaggaa tggcaacgca tagaacagtt
      181 aaggctttct cccctctacg tttccatcca tgccacggaa gcatcggtgc gggaaagatt
      241 gttaaaaaat catcgggccg gtcagattct tgaccagttg gcttggttcc aagctagaag
      301 attacagatc cacgcccagg tggtggtttg tcctggtgtt aatgatggta agcacttaga
      361 gcaaactttg agggacttag cccagtttca ccaaggggaa accccagcgg tgatttcagt
      421 ggcggtggtg ccggtgggtc taacccgttt tcgtccccag gaagatgagt taagccccgt
      481 tggtcaagct aaagccacgg aggtaattgc ccaggttcaa gctttgcaaa aggaatttgc
      541 ccagcaacta ggtggtaact ttgtttggtt ggccgatgaa tggtttctca ttgcccgtca
      601 acctttgcca ccggaaagcc attacgaaga ctatccccaa attggcaacg gggtagggtc
      661 tatccgtcaa ttcattaagg agtttcaaca acaagctgcg gaatttctcc ccccggcgat
      721 cgccgaagcc aagacgttga cctgggtagt gggcaatgct gtagaacaag cgtttgagct
      781 actggtggaa cagttaaatc aggtaaaggg tttaacagtt aatttagcgc ccttaaacag
      841 tgactattgg ggtcaggaaa ttacggtgac gggactattg acggggcagg atttaatcgc
      901 taaattggca ggcagagatt taggggatgg tattctattg cctgctctga tgttgaaaca
      961 tgatgatact cgcttcttgg atgacctccg ggtggccgat gtggctcaaa agttgggcac
     1021 aac
//
