LOCUS       sequence-138150-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Production of Acetobacter aceti 1023 alanine racemase (alr) with 
            N-terminal His tag and Ala365>Asp mutant (H6AaAlr-A365D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Francois JA, Kappock TJ
  TITLE     Alanine racemase from the acidophile Acetobacter aceti.
  JOURNAL   Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6.
  PUBMED    16843006
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2007-01"; volume: "51(1)"; pages: "39-48. Epub 
            2006 Jun 6"
COMMENT     Sequence Label: pJK350
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgtggccag catcgcgcgc aggggctgtg
      121 ttgacgataa atctttccgc tattgccgaa aattaccgct atcttgtgaa caaactgggc
      181 aaaacagttt gcgccgcagt agttaaggcc gatgcttatg ggcttggggt tgcacaggtg
      241 gcgcccattt tggaacaggt tggcgcaaaa catttttttg tcgcacatgt ggatgaaggg
      301 ctagccctta ggccatatct ttcaccccac acatcagtaa ctgtgttgca tggtccacgg
      361 ccggatgctg ctgaggtttg cgtacgcaat aatttgcggc cagttttaaa tagcctaaaa
      421 caggtggctg ttttgcggga tctggcagcc aaaatggata agccgctgga tgtggccatt
      481 caactggata cgggtatgtc tcgttttggc ctttcagagg ctgatatcca taaactgatg
      541 gccactcctg aacttttgga ggggttatct gtcagtctgg ttatgagtca tctggcctgt
      601 gcagatacgc cagaaaacgc agccaatgcc atgcagcgtg aacggtttga gaactatgca
      661 cgcatgttgc caaaggcagc tttaagtctt tctgcatctt ctggaatttt tctggggcaa
      721 ccatttcatt ttgatctcgt gcgccctggt gccgctttgt atggcgtagc gccgcagaat
      781 gatggtgtta atccacttaa gcctgttgtg gggttgcaaa cgcatattct gcaaattcgc
      841 actttatcca aaggggatcg ggtgggatat gggcttacgt ggcaagcgac tggcccatgc
      901 cgtgttgcga ccattgcggt tggatatgct gatggatttg cccgtgcggg cgctgctgca
      961 ggatgcgctt ggtataaagg atatcggcta ccaattctgg gccgaatctc tatggattcc
     1021 atgacagtt
//
