LOCUS       sequence-138220-        7214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the JAM-2 protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Jam2-AP-His
FEATURES             Location/Qualifiers
     source          1..7214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3794..3811
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3837..3958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3874..3893)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3928..3947
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3996..4013)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4167..4755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4247..4266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4926..5717,5718..5786))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5549..5568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5787..5891)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5959..5977
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6015..6037)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6039..6059)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6044..6374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6225..6360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6287..6306
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6511..6530
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6597..7052)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6734..6755)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6946..6965
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7178..7200
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7192..7209
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7193..7209
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcgaggag
     1621 cccccaaggc ctcctgatgc tgctgctgct acactacttg atcgtcgccc tggactatca
     1681 taaggcaaat gggttttctg catcaaaaga ccaccgtcaa gaagtcacag taatagagtt
     1741 ccaagaggct attttggctt gtaaaacccc aaagaagact acctcctcca gactggagtg
     1801 gaagaaggtg ggacaggggg tctccttggt ctactaccaa caggctctcc aaggtgactt
     1861 taaagaccgt gctgagatga tagatttcaa tatacgaatc aaaaatgtta caagaagtga
     1921 tgctggagag tatcgctgtg aagtcagcgc tccgactgag caaggccaga acctgcagga
     1981 agataaagtc atgctagaag tactagtggc tcctgctgtt cctgcctgtg aagtgcccac
     2041 ttctgttatg actggaagtg tggtggagct acgatgccag gataaagaag gaaacccagc
     2101 tccggagtac atctggttta aagatggcac aagtttgcta gggaatccaa aaggcggcac
     2161 acacaacaac agctcgtaca caatgaacac gaagtctgga attctgcaat tcaacatgat
     2221 ttccaagatg gacagtggag agtattactg cgaagcccgg aactctgtcg gacaccgcag
     2281 gtgccctggg aagcgaatgc aagtaggcgc gcctggatct tccggaatca tcccagttga
     2341 ggaggagaac ccggacttct ggaaccgcga ggcagccgag gccctgggtg ccgccaagaa
     2401 gctgcagcct gcacagacag ccgccaagaa cctcatcatc ttcctgggcg atgggatggg
     2461 ggtgtctacg gtgacagctg ccaggatcct aaaagggcag aagaaggaca aactggggcc
     2521 tgagataccc ctggccatgg accgcttccc atatgtggct ctgtccaaga catacaatgt
     2581 agacaaacat gtgccagaca gtggagccac agccacggcc tacctgtgcg gggtcaaggg
     2641 caacttccag accattggct tgagtgcagc cgcccgcttt aaccagtgca acacgacacg
     2701 cggcaacgag gtcatctccg tgatgaatcg ggccaagaaa gcagggaagt cagtgggagt
     2761 ggtaaccacc acacgagtgc agcacgcctc gccagccggc acctacgccc acacggtgaa
     2821 ccgcaactgg tactcggacg ccgacgtgcc tgcctcggcc cgccaggagg ggtgccagga
     2881 catcgctacg cagctcatct ccaacatgga cattgacgtg atcctaggtg ggggccgaaa
     2941 gtacatgttt cgcatgggaa ccccagaccc tgagtaccca gatgactaca gccaaggtgg
     3001 gaccaggctg gacgggaaga atctggtgca ggaatggctg gcgaagcgcc agggtgcccg
     3061 gtatgtgtgg aaccgcactg agctcatgca ggcttccctg gacccgtctg tgacccatct
     3121 catgggtctc tttgagcctg gagacatgaa atacgagatc caccgagact ccacactgga
     3181 cccctccctg atggagatga cagaggctgc cctgcgcctg ctgagcagga acccccgcgg
     3241 cttcttcctc ttcgtggagg gtggtcgcat cgaccatggt catcatgaaa gcagggctta
     3301 ccgggcactg actgagacga tcatgttcga cgacgccatt gagagggcgg gccagctcac
     3361 cagcgaggag gacacgctga gcctcgtcac tgccgaccac tcccacgtct tctccttcgg
     3421 aggctacccc ctgcgaggga gctccatctt cgggctggcc cctggcaagg cccgggacag
     3481 gaaggcctac acggtcctcc tatacggaaa cggtccaggc tatgtgctca aggacggcgc
     3541 ccggccggat gttaccgaga gcgagagcgg gagccccgag tatcggcagc agtcagcagt
     3601 gcccctggac gaagagaccc acgcaggcga ggacgtggcg gtgttcgcgc gcggcccgca
     3661 ggcgcacctg gttcacggcg tgcaggagca gaccttcata gcgcacgtca tggccttcgc
     3721 cgcctgcctg gagccctaca ccgcctgcga cctggcgccc cccgccggca ccaccgacgc
     3781 cgcgcacccg ggtcatcatc accatcacca ttaatctaga gatcttatta aagcagaact
     3841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3901 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3961 atgtctggtc gactctagct agactcttcc gcttcctcgc tcactgactc gctgcgctcg
     4021 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4081 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4141 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4201 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4261 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4321 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4381 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4441 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4501 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4561 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4621 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4681 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4741 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4801 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4861 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4921 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4981 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5041 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5101 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5161 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5221 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5281 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5341 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5401 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5461 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5521 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5581 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5641 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5701 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5761 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5821 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5881 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5941 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     6001 gatgacggtg aaaacctctg acacatgcag ctcccggagg aatgtgtgtc agttagggtg
     6061 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     6121 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6301 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6361 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcgtcccg gagacggtca
     6421 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     6481 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     6541 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     6601 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     6661 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     6721 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     6781 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     6841 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     6901 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     6961 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     7021 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     7081 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     7141 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     7201 acggccagtg aatt
//
