LOCUS       sequence-138246-         592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Production of Acetobacter aceti 1023 alanine racemase (alr) with 
            Tyr260>Ala mutant (AaAlr-Y260A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Francois JA, Kappock TJ
  TITLE     Alanine racemase from the acidophile Acetobacter aceti.
  JOURNAL   Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6.
  PUBMED    16843006
REFERENCE   2  (bases 1 to 592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2007-01"; volume: "51(1)"; pages: "39-48. Epub 
            2006 Jun 6"
COMMENT     Sequence Label: pJK288
FEATURES             Location/Qualifiers
     source          1..592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             32..64
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatggctagc atgactggtg gacagcaaat
       61 gggtcgcgga tccgaattca atgtggccag catcgcgcgc aggggctgtg ttgacgataa
      121 atctttccgc tattgccgaa aattaccgct atcttgtgaa caaactgggc aaaacagttt
      181 gcgccgcagt agttaaggcc gatgcttatg ggcttggggt tgcacaggtg gcgcccattt
      241 tggaacaggt tggcgcaaaa catttttttg tcgcacatgt ggatgaaggg ctagccctta
      301 ggccatatct ttcaccccac acatcagtaa ctgtgttgca tggtccacgg ccggatgctg
      361 ctgaggtttg cgtacgcaat aatttgcggc cagttttaaa tagcctaaaa caggtggctg
      421 ttttgcggga tctggcagcc aaaatggata agccgctgga tgtggccatt caactggata
      481 cgggtatgtc tcgttttggc ctttcagagg ctgatatcca taaactgatg gccactcctg
      541 aacttttgga ggggttatct gtcagtctgg ttatgagtca tctggcctgt gc
//
