LOCUS       sequence-138247-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Production of Acetobacter aceti 1023 alanine racemase (alr) with 
            Tyr260>Ala mutant (AaAlr-Y260A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Francois JA, Kappock TJ
  TITLE     Alanine racemase from the acidophile Acetobacter aceti.
  JOURNAL   Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6.
  PUBMED    16843006
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2007-01"; volume: "51(1)"; pages: "39-48. Epub 
            2006 Jun 6"
COMMENT     Sequence Label: pJK288
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttca ggcagccgtt gcataaacgc gatgataacg ccgccccaga gatgtcagta
      121 cttcgtagcc tattgtgcct tctgctttgg ccacagcatc tacgccgtaa gactcattca
      181 aaagttccac catgctatct tcttccagca gcccttcggg cactgcggaa agatcaactg
      241 tcatggaatc catagagatt cggcccagaa ttggtagccg atatccttta taccaagcgc
      301 atcctgcagc agcgcccgca cgggcaaatc catcagcata tccaaccgca atggtcgcaa
      361 cacggcatgg gccagtcgct tgccacgtaa gcccagctcc cacccgatcc cctttggata
      421 aagtgcgaat ttgcagaata tgcgtttgca accccacaac aggcttaagt ggattaacac
      481 catcattctg cggcgctacg ccatacaaag cggcaccagg gcgcacgaga tcaaaatgaa
      541 atggttgccc cagaaaaatt ccagaagatg cagaaagact taaagctgcc tttggcaaca
      601 tgcgtgcata gttctcaaac cgttcacgct gcatggcatt ggctgcgttt tctggcgtat
      661 ctgcacaggc cagatgactc ataaccagac tgacagataa cccctccaaa agttcaggag
      721 tggccatcag tttatggata tcagcctctg aaaggccaaa acgagacata cccgtatcca
      781 gttgaatggc cacatccagc ggcttatcca ttttggctgc cagatcccgc aaaacagcca
      841 cctgttttag gctatttaaa actggccgca aattattgcg tacgcaaacc tcagcagcat
      901 ccggccgtgg accatgcaac acagttactg atgtgtgggg tgaaagatat ggcctaaggg
      961 ctagcccttc atccacatgt gcgacaaaaa aatgttttgc gccaacctgt tccaaaatgg
     1021 gcgccacctg tgcaacccca agcccataag catcggcctt aactactgcg gcgcaaactg
     1081 ttttgcccag tt
//
