LOCUS       sequence-138298-        1127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For recombinant expression of human GABARAP in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Stangler T, Mayr LM, Dingley AJ, Luge C, Willbold D
  TITLE     Sequence-specific 1H, 13C and 15N resonance assignments of human 
            GABA receptor associated protein.
  JOURNAL   J Biomol NMR. 2001 Oct;21(2):183-4.
  PUBMED    11727985
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomol 
            NMR."; date: "2001-10"; volume: "21(2)"; pages: "183-4"
COMMENT     Sequence Label: pGex-4T-2_GABARAP
FEATURES             Location/Qualifiers
     source          1..1127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..685
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     643..665
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             692..709
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ggataacaat ttcacmcagg aaacagtatt catgtcccct atactaggtt attggaaaat
       61 taagggcctt gtgcaaccca ctcgacttct tttggaatat cttgaagaaa aatatgaaga
      121 gcatttgtat gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg aattgggttt
      181 ggagtttccc aatcttcctt attatattga tggtgatgtt aaattaacac agtctatggc
      241 catcatacgt tatatagctg acaagcacaa catgttgggt ggttgtccaa aagagcgtgc
      301 agagatttca atgcttgaag gagcggtttt ggatattaga tacggtgttt cgagaattgc
      361 atatagtaaa gactttgaaa ctctcaaagt tgattttctt agcaagctac ctgaaatgct
      421 gaaaatgttc gaagatcgtt tatgtcataa aacatattta aatggtgatc atgtaaccca
      481 tcctgacttc atgttgtatg acgctcttga tgttgtttta tacatggacc caatgtgcct
      541 ggatgcgttc ccaaaattag tttgttttaa aaaacgtatt gaagctatcc cacaaattga
      601 taagtacttg aaatccagca agtatatagc atggcctttg cagggctggc aagccacgtt
      661 tggtggtggc gaccatcctc caaaatcgga tctggttccg cgtggatcca tgaagttcgt
      721 gtacaaagaa gagcatccgt tcgagaagcg ccgctctgag ggcgagaaga tccgaaagaa
      781 atacccggac cgggtgccgg tgatagtaga aaaggctccc aaagctcgga taggagacct
      841 ggacaaaaag aaatacctgg tgccttctga tctcacagtt ggtcagttct acttcttgat
      901 ccggaagcga attcatctcc gagctgagga tgccttgttt ttctttgtca acaatgtcat
      961 tccacccacc agtgccacaa tgggtcagct gtaccaggaa caccatgaag aagacttctt
     1021 tctctacatt gcctacagtg acgaaagtgt ctacggtctg taatgcggcc gcatcgtgac
     1081 tgactgacga tctgcctcgc gcgtttcggt gatgacggtg aaaacct
//
