LOCUS       sequence-138317-        5046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Conklin BR, Farfel Z, Lustig KD, Julius D, Bourne HR
  TITLE     Substitution of three amino acids switches receptor specificity of 
            Gq alpha to that of Gi alpha.
  JOURNAL   Nature. 1993 May 20. 363(6426):274-6.
  PUBMED    8387644
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1993 May 20. 363(6426):274-6."
COMMENT     Sequence Label: qo5
FEATURES             Location/Qualifiers
     source          1..5046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        389..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     543..563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     637..656
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        637..655
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1786..1804)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1787..1804)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          1888..1953
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2083..2103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2375..2509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2412..2431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2466..2485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3360..3495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3990..4009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(4122..4580)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4259..4280)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4474..4493
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     tRNA            complement(join(4703..4722,4723..4807,4808..4882))
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
ORIGIN
        1 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
      601 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      661 ccaagcttgg taccgagctc ggatccatga ctctggagtc catcatggca tgctgcctga
      721 gcgaggaggc caaggaagcc cggaggatca acgacgagat cgagcggcag ctgcgcaggg
      781 acaagcgcga cgcccgccgg gagctcaagc tgctgctgct ggggacaggg gagagtggca
      841 agtcgacctt catcaagcag atgaggatca tccacgggtc gggctactct gacgaagaca
      901 agcgcggctt caccaagctg gtgtatcaga acatcttcac ggccatgcag gccatgatca
      961 gagcgatgga cacactcaag atcccataca agtatgaaca caataaggct catgcacaat
     1021 tggttcgaga ggttgatgtg gagaaggtgt ctgcttttga cgtccccgac tacgcggcaa
     1081 taaagagctt gtggaatgat cctggaatcc aggagtgcta cgacagacga cgggaatatc
     1141 agttatctga ctctaccaaa tactatctga atgacttgga ccgtgtagcc gacccttcct
     1201 atctgcctac acaacaagac gtgcttagag ttcgagtccc cactacaggg atcatcgaat
     1261 acccctttga cttacaaagt gtcattttca gaatggtcga tgtagggggc caaaggtcag
     1321 agagaagaaa atggatacac tgctttgaaa atgtcacctc catcatgttt ctagtagcgc
     1381 ttagcgaata tgatcaagtt cttgtggagt cagacaatga gaaccgcatg gaggagagca
     1441 aagcactctt tagaacaatt atcacctacc cctggttcca gaactcctct gtgattctgt
     1501 tcttaaacaa gaaagatctt ctagaggaga aaatcatgta ttcccaccta gtcgactact
     1561 tcccagaata tgatggaccc cagagagatg cccaggcagc tcgagaattc atcctgaaaa
     1621 tgttcgtgga cctgaacccc gacagtgaca aaatcatcta ctcccacttc acgtgcgcca
     1681 cagataccga gaacatccgc ttcgtctttg cagccgtcaa ggacaccatc ctgcagctga
     1741 acctgaaggg ctgtggcttg tactaatgca tctagagggc cctattctat agtgtcacct
     1801 aaatgctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     1861 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     1921 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     1981 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     2041 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     2101 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     2161 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     2221 tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga tcataatcag
     2281 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2341 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2401 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2461 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg atcccgccat
     2521 ggtatcaacg ccatatttct atttacagta gggacctctt cgttgtgtag gtaccgctgt
     2581 attcctaggg aaatagtaga ggcaccttga actgtctgca tcagccatat agcccccgct
     2641 gttcgattta caaacacagg cacagtactg acaaacccat acacctcctc tgaaataccc
     2701 atagttgcta gggctgtctc cgaactcatt acaccctcca aagtcagagc tgtaatttcg
     2761 ccatcaaggg cagcgagggc ttctccagat aaaatagctt ctgccgagag tcccgtaagg
     2821 gtagacactt cagctaatcc ctcgatgagg tctactagaa tagtcagtgc ggctcccatt
     2881 ttgaaaattc acttacttga tcagcttcag aagatggcgg agggcctcca acacagtaat
     2941 tttcctcccg actcttaaaa tagaaaatgt caagtcagtt aagcaggaag tgactaactg
     3001 accgcagctg gccgtgcgac atcctctttt aattagttgc taggcaactg ccctccagag
     3061 ggcagtgtgg ttttgcaaga ggaagcaaaa agcctctcca cccaggccta gaatgtttcc
     3121 acccaatcat tactatgaca acagctgttt tttttagtat taagcagagg ccgggggccc
     3181 ctggcctccg cttactctgg agaaaaagaa gagaggcatt gtagaggctt ccagaggcaa
     3241 cttgtcaaaa caggactgct tctatttctg tcacactgtc tggccctgtc acaaggtcca
     3301 gcacctccat acccccttta ataagcagtt tgggaacggg tgcgggtctt actccgccca
     3361 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3421 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     3481 gcttttttgg aggcctaggc ttttgcaaaa agctaattcg gcgtaatctg ctgcttgcaa
     3541 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3601 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3661 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgctg
     3721 aagccagtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3781 aagagatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3841 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3901 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3961 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4021 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4081 ctatggaaaa acgccagcaa cgcaagctag cttctagcta gaaattgtaa acgttaatat
     4141 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4201 aatcggcaaa atcccttata aatcaaaaga atagcccgag atagggttga gtgttgttcc
     4261 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4321 cgtctatcag ggcgatggcc gcccactacg tgaaccatca cccaaatcaa gttttttggg
     4381 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4441 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4501 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     4561 tgcgccgcta cagggcgcgt actatggttg ctttgacgag cacgtataac gtgctttcct
     4621 cgttagaatc agagcgggag ctaaacagga ggccgattaa agggatttta gacaggaacg
     4681 gtacgccagc tggaccgcgg tctttcggac ttttgaaagt gatggtggtg ggggaaggat
     4741 tcgaaccttc gaagtcgatg acggcagatt tagagtctgc tccctttggc cgctcgggaa
     4801 ccccaccaca ggtaatgctt ttactggcct gctcccttat cgggaagcgg ggcgcatcat
     4861 atcaaatgac gcgccgctgt aaagtgttac gttgagaaag ctgctccctg cttgtgtgtt
     4921 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     4981 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     5041 cagata
//
