LOCUS       sequence-138405-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase, His59>Asp/His89>Asp mutant 
            (AaPurE1-H59D,H89D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK294
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             624..641
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(694..712)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      708..755
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(879..898)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgatgagc gaaaccgccc ccctgccctc
       61 tgcttccagt gctttagaag ataaagcagc ctctgccccc gttgtgggca ttatcatggg
      121 cagccagtca gactgggaaa ccatgcgcca tgcagacgca ttgctgacag agctggaaat
      181 tccgcatgaa accctcattg tttctgcaga tcgtacgcca gaccggttgg cggattatgc
      241 gcgtacggca gccgaacgcg gcctgaatgt gattattgct ggggccggtg gagctgcaga
      301 tctgccgggt atgtgtgcgg catggacacg cctgcccgtg ctgggtgttc cagtagaatc
      361 tcgcgctctt aaaggcatgg acagcctgct ttccattgtg cagatgcccg gcggcgtgcc
      421 tgtgggcact ttggctattg gggcatccgg cgccaaaaat gccgccttgc tggccgcctc
      481 cattcttgca ctgtataacc cggcccttgc cgcacgcctt gaaacatggc gcgcattgca
      541 gacggcctct gtccccaact ctcccattac cgaagataaa tgagaattcg agctccgtcg
      601 acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca
      661 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      721 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      781 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      841 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      901 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      961 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     1021 acgta
//
