LOCUS       sequence-138564-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide 
            mutase (EcPurE1); NCAIR synthetase (EcPurK).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Meyer E, Kappock TJ, Osuji C, Stubbe J
  TITLE     Evidence for the direct transfer of the carboxylate of 
            N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to generate 
            4-carboxy-5-aminoimidazole ribonucleotide catalyzed by Escherichia 
            coli PurE, an N5-CAIR mutase.
  JOURNAL   Biochemistry. 1999 Mar 9;38(10):3012-8.
  PUBMED    10074353
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "1999-03-9"; volume: "38(10)"; pages: 
            "3012-8"
COMMENT     Sequence Label: pNC2
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgc atgcctgcag ttaaccgaac ttactctgcg cccaaatcac gccgctggca
      121 tattccggcg gcagcagcgg gattaaggct tccagcgtcg cagtcagacg cgatgtgtcg
      181 ctgtcggtca aattcagatg ccccacttta cgccccggac ggacttcttt gtcgtaccag
      241 tgcagatgca ccagcggcag tttcagccag tcataattca catcgctacc aatcagattg
      301 atcatcaccg acggattatt caccactggt tgcggtaacg gcagatcggt aatcgcccgc
      361 agatgcagct caaactggct gatgctggca ccgttttgtg tccagtgacc gctgttatgc
      421 acacgcggtg ccagttcgtt gatcaacaga ccttgcgggg tgacaaaaca ctccatcgcc
      481 atcacgccca catagcccag ctcctgcata atcgccgaca gcatctcttc ggctcgcgcc
      541 tgctgctgtg cgttggcctg cggaaaagcg acgctggtgc gcaaaatacc gtcctgatgc
      601 aggttatgcg tcagcggata aaacacggtg ctgccatcaa agccgcgcgc gccaaccagc
      661 gacacttcac cagagaagtt aatgccctgc tcgacaatac attcgccgta acactctgcc
      721 ggtaactgtt cggtttcatt tgcgcgtaaa cgccattgac cgcgaccgtc ataaccacca
      781 gtgcgacgct taacaatcgc cagctcacct aaacgatcaa acaccgcagg ccactcgctg
      841 cgttcggcaa gtaactgcca cggtgcagtc ggcaggtgga gcttatcgaa aagctgcttc
      901 tgagtcagac ggtcagcaat aatcgggaac acatcgcggt tcacaaaggc cggatggcgc
      961 gccagctcgc gggttaatgc ggtttccggc cagcgttcta tctcagcggt aatcacgctt
     1021 tgttgaaaag gcaccg
//
