LOCUS       sequence-138670-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Sema3A protein (truncated at cleavage site P3; ie, 
            long and missing exon 3), C-terminally fused to the Fc region of 
            human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3a(L, del3)-Fc-His
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ataagatctc tagattaatg gtgatggtga tgatggccgc ctttacccgg agacagggag
       61 aggctcttct gcgtgtagtg gttgtgcaga gcctcatgca tcacggagca tgagaagacg
      121 ttcccctgct gccacctgct cttgtccacg gtgagcttgc tgtagaggaa gaaggagccg
      181 tcggagtcca gcacgggagg cgtggtcttg tagttgttct ccggctgccc attgctctcc
      241 cactccacgg cgatgtcgct gggatagaag cctttgacca ggcaggtcag gctgacctgg
      301 ttcttggtca gctcatcccg ggatgggggc agggtataca cctgtggttc tcggggctgc
      361 cctttggctt tggagatggt tttctcgatg ggggctggga gggctttgtt ggagaccttg
      421 cacttgtact ccttgccatt cagccagtcc tggtgcagga cggtgaggac gctgaccaca
      481 cggtacgtgc tgttgtactg ctcctcccgc ggctttgtct tggcattatg cacctccacg
      541 ccgtccacgt accagttgaa cttgacctca gggtcttcgt ggctcacgtc caccaccacg
      601 catgtgacct caggggtccg ggagatcatg agggtgtcct tgggttttgg ggggaagagg
      661 aagactgacg gtccccccag gagttcaggt gctgggcacg gtgggcatgt gtgagttttg
      721 tcaggcgcgc cgggtgcccg ctcaaactcg tgcgtacgcc tgtttctacc tttcttgctc
      781 tcttgcatgt gcttccactt gttgctgctc ccttgagagt gccccggcct ttgtcggcgt
      841 tgctttcggt ccctt
//
