LOCUS       sequence-138790-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PH domain for phosphoinositide binding 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Yu JW, Mendrola JM, Audhya A, Singh S, Keleti D, DeWald DB, Murray 
            D, Emr SD, Lemmon MA
  TITLE     Genome-wide analysis of membrane targeting by S. cerevisiae 
            pleckstrin homology domains.
  JOURNAL   Mol Cell. 2004 Mar 12;13(5):677-88.
  PUBMED    15023338
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2004-03-12"; volume: "13(5)"; pages: "677-88"
COMMENT     Sequence Label: pGEX2TK-Num1-PH
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(430..447)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(474..496)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tcagtcacga tgaattcact ctaaacttat cccttgcatg ttcctttgaa gtaaataacg
       61 taatgaatta taccaaatat tgtgtctttg ccttgtagga caagtaaact taatagtcct
      121 agtttctgtg gtaacaacaa tacttttgtg atacaatcct gttggatatg ggtttgggtc
      181 tgtgacactt tctactccta gaatggcaac accttttgtt ttggtattgg caggattctc
      241 taggatggga ttagaagcgg accagtacaa agttaaggta tatggatgaa cccagaagaa
      301 tctttcatga cgtgattcga atccaaaagg tcccaagcgt ggataatact taaacaaata
      361 ttccccaatg acggtttggg ttaacgcggg tattatgctt ggttcgttgg atccaacaga
      421 tgcacgacga gatccacgcg gaaccagatc cgattttgga ggatggtcgc caccaccaaa
      481 cgtggcttgc cagccctgca aaggccatgc tatatacttg ctggatttca agtacttatc
      541 aatttgtggg atagcttcaa tacgtttttt aaaacaaact aattttggga acgcatccag
      601 gcacattggg tccatgtata aaacaacatc aagagcgtca tacaacatga agtcaggatg
      661 ggttacatga tcaccattta aatatgtttt atgacataaa cgatcttcga acattttcag
      721 catttcaggt agcttgctaa gaa
//
