LOCUS       sequence-13881-e        2805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pAd:TRE-gateway
FEATURES             Location/Qualifiers
     source          1..2805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(53..72)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    join(217..235,236..258,259..277,278..300,301..319,320..342,
                     343..361,362..384,385..403,404..426,427..445,446..468,
                     469..487)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        520..557
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     554..578
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    771..895
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(920..925,926..943,944..950)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1004..1663
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1070..1089)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2005..2310
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2189..2208
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2351..2475)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2493..2510)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2499..2723
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggtagcgaaa gctcagatct ggatctcccg atcccctatg gtcgactctc agtacaatct
       61 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
      121 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgatagg
      181 cgtatcacga ggccctttcg tcctcgagtt taccactccc tatcagtgat agagaaaagt
      241 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
      301 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      361 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      421 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      481 atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag taggcgtgta cggtgggagg
      541 cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
      601 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcgagc
      661 tcggtacccg gggatcctct agaggatcca gacatgataa gggccctcta gactcgagcg
      721 gccgccactg tgctggatga tccgagctcg gtaccaagct taagtttatc acaagtttgt
      781 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
      841 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
      901 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
      961 gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1021 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1081 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1141 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1201 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1261 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1321 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1381 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1441 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1501 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1561 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
     1621 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt
     1681 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     1741 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
     1801 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     1861 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     1921 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     1981 ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2041 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2101 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2161 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2221 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2281 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2341 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2401 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2461 cttgtacaaa gtggtgataa accgctgatc agcctcgact gtgccttcta gttgccagcc
     2521 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     2581 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     2641 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     2701 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagcagat ctgcagatct
     2761 gaattcatct atgtcgggtg cggagaaaga ggtaatgaaa tggca
//
