LOCUS       sequence-138888-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a 
            fusion to Pas2p, with c-myc tag and His6-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK142
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..74)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(90..119)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ttctcgtaag tgcccaactt gaactgagga acagtcatgt ctaaggctaa aactcaatga
       61 tgatgatgat gatggtcgac ggcgctattc agatcctctt ctgagatgag tttttgttcg
      121 ggccccgcga atttgttgta ccagtaaaca cctttagact ctctgccttt ctctggctcg
      181 atgaagatgc actccttagc ctcacgccac atggctttca atattggagt tctatcgatt
      241 ttgtagaagt cgcccagaac agtgttgata gcgtctctag cctccttagc gtggtagtgc
      301 gggatgtagc tgaacaagtg atgcattacg tgggtgtggg taacatcgtg cagaacagag
      361 ttcaggaaac cgaagtctct gtcgatggta gatagagctc cacgcagcca gttccactca
      421 gaagagtcgt agtgtggcaa ggacaggtgg gtgtggtgca ggtaagtgat gatatcgaag
      481 aagatgaaca cgcctagaac cgggatccca tagatgcagg ttacccaagc ggcaccctta
      541 gcagcaacag ccaatttaac accgtataaa acagctagta gacctaggtc ggacagcaga
      601 acctggaatc tctctctctc cttgaagatt ggactcatcg gatcgaagtg gttagcgaaa
      661 cgctcgtatt ttttaccaga gatgttggtg aagaggtata atgggaaacc taaagtgaag
      721 gtgatgaaca tgattaataa acgacccggg gggtggttca gaactttata gtacagagct
      781 actttggctt tagatttcgg gatgtaaacc tcatcgttgt ccagagaatt cgtgttagcg
      841 tggtggttac ggtgagagta tttccagctg aagtatggag tcattaggaa agagtgcaaa
      901 atgaaaccaa cggtgtcgtc tacccactgg tagtcagaga acgcgtggtg accgcactcg
      961 tggccgatta cccacagacc ggtcaggata gaagcttgac agaaccaata cagcggccaa
     1021 gctaggtagg ctagcggggc tggc
//
